1GHS
THE THREE-DIMENSIONAL STRUCTURES OF TWO PLANT BETA-GLUCAN ENDOHYDROLASES WITH DISTINCT SUBSTRATE SPECIFICITIES
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004553 | molecular_function | hydrolase activity, hydrolyzing O-glycosyl compounds |
| A | 0005975 | biological_process | carbohydrate metabolic process |
| A | 0006076 | biological_process | (1->3)-beta-D-glucan catabolic process |
| A | 0006952 | biological_process | defense response |
| A | 0016798 | molecular_function | hydrolase activity, acting on glycosyl bonds |
| A | 0042973 | molecular_function | glucan endo-1,3-beta-D-glucosidase activity |
| A | 0050832 | biological_process | defense response to fungus |
| B | 0004553 | molecular_function | hydrolase activity, hydrolyzing O-glycosyl compounds |
| B | 0005975 | biological_process | carbohydrate metabolic process |
| B | 0006076 | biological_process | (1->3)-beta-D-glucan catabolic process |
| B | 0006952 | biological_process | defense response |
| B | 0016798 | molecular_function | hydrolase activity, acting on glycosyl bonds |
| B | 0042973 | molecular_function | glucan endo-1,3-beta-D-glucosidase activity |
| B | 0050832 | biological_process | defense response to fungus |
Functional Information from PROSITE/UniProt
| site_id | PS00587 |
| Number of Residues | 14 |
| Details | GLYCOSYL_HYDROL_F17 Glycosyl hydrolases family 17 signature. VkVVVSESGWPSaG |
| Chain | Residue | Details |
| A | VAL225-GLY238 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: Proton donor => ECO:0000250|UniProtKB:O22317 |
| Chain | Residue | Details |
| A | GLU94 | |
| B | GLU94 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | ACT_SITE: Nucleophile => ECO:0000250|UniProtKB:O22317 |
| Chain | Residue | Details |
| A | GLU231 | |
| B | GLU231 |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 4 |
| Details | M-CSA 580 |
| Chain | Residue | Details |
| A | GLU231 | covalently attached, electrostatic stabiliser, nucleofuge, nucleophile |
| A | GLU279 | activator, modifies pKa |
| A | LYS282 | activator, modifies pKa |
| A | GLU288 | activator, increase nucleophilicity, proton acceptor, proton donor |
| site_id | MCSA2 |
| Number of Residues | 4 |
| Details | M-CSA 580 |
| Chain | Residue | Details |
| B | GLU231 | covalently attached, electrostatic stabiliser, nucleofuge, nucleophile |
| B | GLU279 | activator, modifies pKa |
| B | LYS282 | activator, modifies pKa |
| B | GLU288 | activator, increase nucleophilicity, proton acceptor, proton donor |
