1GHS

THE THREE-DIMENSIONAL STRUCTURES OF TWO PLANT BETA-GLUCAN ENDOHYDROLASES WITH DISTINCT SUBSTRATE SPECIFICITIES

Experimental procedure

Spacegroup name	P 32 2 1
Unit cell lengths	86.900, 86.900, 156.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.300
R-factor	0.179
Rwork	0.179
RMSD bond length	0.013
RMSD bond angle	1.680
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.800 *
Rmerge	0.098 *
Total number of observations	114164 *
Number of reflections	32509 *
Completeness [%]	78.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	10 (mg/ml)
2	1	drop	ammonium sulfate	15 (%sat)
3	1	drop	Tris-HCl	100 (mM)
4	1	reservoir	ammonium sulfate	35 (%sat)