1GHB

A SECOND ACTIVE SITE IN CHYMOTRYPSIN? THE X-RAY CRYSTAL STRUCTURE OF N-ACETYL-D-TRYPTOPHAN BOUND TO GAMMA-CHYMOTRYPSIN

Functional Information from GO Data

Chain	GOid	namespace	contents
F	0004252	molecular_function	serine-type endopeptidase activity
F	0006508	biological_process	proteolysis
G	0004252	molecular_function	serine-type endopeptidase activity
G	0006508	biological_process	proteolysis

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE ACE E 600

Chain	Residue
E	TRP601
G	SER189
G	SER190
G	GLY216
G	SER217
G	GLY226
G	HOH717

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site_id	AC2
Number of Residues	14
Details	BINDING SITE FOR RESIDUE TRP E 601

Chain	Residue
G	MET192
G	SER195
G	VAL213
G	SER214
G	TRP215
G	GLY216
G	SER217
G	CYS220
G	GLY226
G	VAL227
G	TYR228
P	ALA573
E	ACE600
G	SER190

---

site_id	AC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE HEX E 651

Chain	Residue
F	ILE16
F	ASN18
F	GLY19
F	THR144
G	GLN156

---

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE HEX G 652

Chain	Residue
E	HOH659
F	GLU20
G	SER159
G	SER186
G	GLY187

---

site_id	AC5
Number of Residues	8
Details	BINDING SITE FOR RESIDUE HEX E 653

Chain	Residue
E	CYS1
E	VAL3
F	THR37
F	LYS93
F	SER119
F	ALA120
F	HOH709
F	HOH869

---

site_id	AC6
Number of Residues	3
Details	BINDING SITE FOR RESIDUE HEX F 654

Chain	Residue
E	HOH676
F	GLU20
F	GLU21

---

site_id	AC7
Number of Residues	1
Details	BINDING SITE FOR RESIDUE HEX F 655

Chain	Residue
F	GLU49

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site_id	AC8
Number of Residues	7
Details	BINDING SITE FOR RESIDUE IPA G 701

Chain	Residue
F	PHE41
F	SER96
F	LEU97
G	MET192
G	GLY193
G	SER195
P	ALA573

---

site_id	AC9
Number of Residues	2
Details	BINDING SITE FOR RESIDUE IPA G 702

Chain	Residue
G	SER218
G	HOH769

---

site_id	BC1
Number of Residues	8
Details	BINDING SITE FOR RESIDUE IPA F 703

Chain	Residue
F	ALA56
F	GLY59
F	GLY59
F	VAL60
F	THR61
F	VAL88
F	LYS90
F	THR104

---

site_id	BC2
Number of Residues	11
Details	BINDING SITE FOR CHAIN P OF PRO-GLY-ALA

Chain	Residue
E	TRP601
F	HIS57
F	LEU97
F	ILE99
F	HOH737
G	SER195
G	SER214
G	TRP215
G	GLY216
G	IPA701
P	HOH574

---

Functional Information from PROSITE/UniProt

site_id	PS00134
Number of Residues	6
Details	TRYPSIN_HIS Serine proteases, trypsin family, histidine active site. VTAAHC

Chain	Residue	Details
F	VAL53-CYS58

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site_id	PS00135
Number of Residues	12
Details	TRYPSIN_SER Serine proteases, trypsin family, serine active site. SScmGDSGGPLV

Chain	Residue	Details
G	SER189-VAL200

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Charge relay system

Chain	Residue	Details
G	SER195
F	ASP102

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Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1hja

Chain	Residue	Details
E	NAL *
E	NAL *

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site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1hja

Chain	Residue	Details
E	NAL *
E	NAL *

---

site_id	CSA3
Number of Residues	2
Details	Annotated By Reference To The Literature 1hja

Chain	Residue	Details
E	NAL *
E	NAL *

---

site_id	CSA4
Number of Residues	2
Details	Annotated By Reference To The Literature 1hja

Chain	Residue	Details
F	ASP102
F	HIS57

---

site_id	CSA5
Number of Residues	2
Details	Annotated By Reference To The Literature 1hja

Chain	Residue	Details
G	SER195
G	GLY193

---

site_id	CSA6
Number of Residues	2
Details	Annotated By Reference To The Literature 1hja

Chain	Residue	Details
G	SER195
G	GLY196

---

site_id	MCSA1
Number of Residues	3
Details	M-CSA 387

Chain	Residue	Details
G	GLY193	electrostatic stabiliser
G	SER195	covalent catalysis
G	GLY196	electrostatic stabiliser

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