1GHB

A SECOND ACTIVE SITE IN CHYMOTRYPSIN? THE X-RAY CRYSTAL STRUCTURE OF N-ACETYL-D-TRYPTOPHAN BOUND TO GAMMA-CHYMOTRYPSIN

Experimental procedure

Spacegroup name	P 42 21 2
Unit cell lengths	69.600, 69.600, 97.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	5.000 * - 2.200 *
R-factor	0.152
Rwork	0.152
RMSD bond length	0.010
RMSD bond angle	1.606
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.200 *
Rmerge	0.140 *
Number of reflections	15361 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	5.6 *		Yennawar, N. H., (1994) Biochemistry, 33, 7326. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	chymotrypsin	15 (mg/ml)
2	1	1	sodium cacodylate	10 (mM)
3	1	1	cetyltrimethylammonium bromide	0.75 (%sat)
4	1	1	ammonium sulfate	45 (%sat)
5	1	2	ammonium sulphate	65 (%sat)