Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

1FQJ

CRYSTAL STRUCTURE OF THE HETEROTRIMERIC COMPLEX OF THE RGS DOMAIN OF RGS9, THE GAMMA SUBUNIT OF PHOSPHODIESTERASE AND THE GT/I1 CHIMERA ALPHA SUBUNIT [(RGS9)-(PDEGAMMA)-(GT/I1ALPHA)-(GDP)-(ALF4-)-(MG2+)]

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003924	molecular_function	GTPase activity
A	0005525	molecular_function	GTP binding
A	0007165	biological_process	signal transduction
A	0007186	biological_process	G protein-coupled receptor signaling pathway
A	0007188	biological_process	adenylate cyclase-modulating G protein-coupled receptor signaling pathway
A	0019001	molecular_function	guanyl nucleotide binding
A	0031683	molecular_function	G-protein beta/gamma-subunit complex binding
B	0008277	biological_process	regulation of G protein-coupled receptor signaling pathway
C	0004114	molecular_function	3',5'-cyclic-nucleotide phosphodiesterase activity
C	0007601	biological_process	visual perception
C	0030553	molecular_function	cGMP binding
D	0003924	molecular_function	GTPase activity
D	0005525	molecular_function	GTP binding
D	0007165	biological_process	signal transduction
D	0007186	biological_process	G protein-coupled receptor signaling pathway
D	0007188	biological_process	adenylate cyclase-modulating G protein-coupled receptor signaling pathway
D	0019001	molecular_function	guanyl nucleotide binding
D	0031683	molecular_function	G-protein beta/gamma-subunit complex binding
E	0008277	biological_process	regulation of G protein-coupled receptor signaling pathway

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG A 352

Chain	Residue
A	SER43
A	THR177
A	GDP360
A	ALF362
A	HOH364
A	HOH365

site_id	AC2
Number of Residues	15
Details	BINDING SITE FOR RESIDUE ALF A 362

Chain	Residue
A	ARG174
A	VAL175
A	LYS176
A	THR177
A	VAL197
A	GLY199
A	GLN200
A	MG352
A	GDP360
A	HOH363
A	HOH364
A	HOH365
A	GLY38
A	GLU39
A	LYS42

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG D 352

Chain	Residue
D	SER43
D	THR177
D	GDP361
D	ALF363
D	HOH365
D	HOH366

site_id	AC4
Number of Residues	15
Details	BINDING SITE FOR RESIDUE ALF D 363

Chain	Residue
D	GLY38
D	GLU39
D	LYS42
D	ARG174
D	VAL175
D	LYS176
D	THR177
D	VAL197
D	GLY199
D	GLN200
D	MG352
D	GDP361
D	HOH364
D	HOH365
D	HOH366

site_id	AC5
Number of Residues	25
Details	BINDING SITE FOR RESIDUE GDP A 360

Chain	Residue
A	GLU39
A	SER40
A	GLY41
A	LYS42
A	SER43
A	THR44
A	ASP146
A	SER147
A	LEU171
A	ARG172
A	SER173
A	ARG174
A	ASN265
A	LYS266
A	ASP268
A	LEU269
A	CYS321
A	ALA322
A	THR323
A	MG352
A	ALF362
A	HOH364
A	HOH366
A	HOH368
A	HOH374

site_id	AC6
Number of Residues	26
Details	BINDING SITE FOR RESIDUE GDP D 361

Chain	Residue
D	GLY38
D	GLU39
D	SER40
D	GLY41
D	LYS42
D	SER43
D	THR44
D	ASP146
D	SER147
D	LEU171
D	ARG172
D	SER173
D	ARG174
D	ASN265
D	LYS266
D	ASP268
D	LEU269
D	CYS321
D	ALA322
D	THR323
D	MG352
D	ALF363
D	HOH365
D	HOH368
D	HOH369
D	HOH373

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	26
Details	Region: {"description":"G1 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	16
Details	Region: {"description":"G2 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	18
Details	Region: {"description":"G3 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	14
Details	Region: {"description":"G4 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	10
Details	Region: {"description":"G5 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	28
Details	Binding site: {"evidences":[{"source":"PubMed","id":"7969474","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8208289","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8259210","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1FQJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1FQK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GOT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1TAD","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1TAG","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1TND","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3V00","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"25037222","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9705312","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1BH2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4PAO","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"19703466","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"25037222","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9705312","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1BH2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4PAO","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI9
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"8259210","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1TND","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI10
Number of Residues	30
Details	Binding site: {"evidences":[{"source":"PDB","id":"1AS0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1BH2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GIA","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GIL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3FFA","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4N0D","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4PAO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4PAQ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI11
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PDB","id":"1AS0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GIA","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GIL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3FFA","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4N0D","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4PAO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4PAQ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI12
Number of Residues	2
Details	Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"UniProtKB","id":"P11488","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI13
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PDB","id":"1AS0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1BH2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GIL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4N0D","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI14
Number of Residues	230
Details	Domain: {"description":"RGS","evidences":[{"source":"PROSITE-ProRule","id":"PRU00171","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

Catalytic Information from CSA

site_id	CSA1
Number of Residues	4
Details	Annotated By Reference To The Literature 1ksj

Chain	Residue	Details
A	THR177
A	GLN200
A	GLU39
A	ARG174

site_id	CSA2
Number of Residues	4
Details	Annotated By Reference To The Literature 1ksj

Chain	Residue	Details
D	THR177
D	GLN200
D	GLU39
D	ARG174

site_id	CSA3
Number of Residues	1
Details	Annotated By Reference To The Literature 1ksj

Chain	Residue	Details
A	GLN200

site_id	CSA4
Number of Residues	1
Details	Annotated By Reference To The Literature 1ksj

Chain	Residue	Details
D	GLN200

site_id	MCSA1
Number of Residues	5
Details	M-CSA 533

Chain	Residue	Details
A	GLU39	electrostatic stabiliser
A	THR44	electrostatic stabiliser
A	ARG174	electrostatic stabiliser
A	ASP196	electrostatic stabiliser
A	GLN200	electrostatic stabiliser

site_id	MCSA2
Number of Residues	5
Details	M-CSA 533

Chain	Residue	Details
D	GLU39	electrostatic stabiliser
D	THR44	electrostatic stabiliser
D	ARG174	electrostatic stabiliser
D	ASP196	electrostatic stabiliser
D	GLN200	electrostatic stabiliser

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)