Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

1FDV

HUMAN 17-BETA-HYDROXYSTEROID-DEHYDROGENASE TYPE 1 MUTANT H221L COMPLEXED WITH NAD+

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0004303	molecular_function	estradiol 17-beta-dehydrogenase [NAD(P)] activity
A	0005496	molecular_function	steroid binding
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006629	biological_process	lipid metabolic process
A	0006694	biological_process	steroid biosynthetic process
A	0006703	biological_process	estrogen biosynthetic process
A	0007040	biological_process	lysosome organization
A	0007519	biological_process	skeletal muscle tissue development
A	0008210	biological_process	estrogen metabolic process
A	0010467	biological_process	gene expression
A	0016491	molecular_function	oxidoreductase activity
A	0030283	molecular_function	testosterone dehydrogenase [NAD(P)] activity
A	0035410	molecular_function	dihydrotestosterone 17-beta-dehydrogenase activity
A	0036094	molecular_function	small molecule binding
A	0042803	molecular_function	protein homodimerization activity
A	0047035	molecular_function	testosterone dehydrogenase (NAD+) activity
A	0047045	molecular_function	testosterone 17-beta-dehydrogenase (NADP+) activity
A	0050661	molecular_function	NADP binding
A	0060348	biological_process	bone development
A	0060612	biological_process	adipose tissue development
A	0061370	biological_process	testosterone biosynthetic process
A	0070401	molecular_function	NADP+ binding
A	0071248	biological_process	cellular response to metal ion
A	0072582	molecular_function	17-beta-hydroxysteroid dehydrogenase (NADP+) activity
A	1903924	molecular_function	estradiol binding
B	0003824	molecular_function	catalytic activity
B	0004303	molecular_function	estradiol 17-beta-dehydrogenase [NAD(P)] activity
B	0005496	molecular_function	steroid binding
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006629	biological_process	lipid metabolic process
B	0006694	biological_process	steroid biosynthetic process
B	0006703	biological_process	estrogen biosynthetic process
B	0007040	biological_process	lysosome organization
B	0007519	biological_process	skeletal muscle tissue development
B	0008210	biological_process	estrogen metabolic process
B	0010467	biological_process	gene expression
B	0016491	molecular_function	oxidoreductase activity
B	0030283	molecular_function	testosterone dehydrogenase [NAD(P)] activity
B	0035410	molecular_function	dihydrotestosterone 17-beta-dehydrogenase activity
B	0036094	molecular_function	small molecule binding
B	0042803	molecular_function	protein homodimerization activity
B	0047035	molecular_function	testosterone dehydrogenase (NAD+) activity
B	0047045	molecular_function	testosterone 17-beta-dehydrogenase (NADP+) activity
B	0050661	molecular_function	NADP binding
B	0060348	biological_process	bone development
B	0060612	biological_process	adipose tissue development
B	0061370	biological_process	testosterone biosynthetic process
B	0070401	molecular_function	NADP+ binding
B	0071248	biological_process	cellular response to metal ion
B	0072582	molecular_function	17-beta-hydroxysteroid dehydrogenase (NADP+) activity
B	1903924	molecular_function	estradiol binding
C	0003824	molecular_function	catalytic activity
C	0004303	molecular_function	estradiol 17-beta-dehydrogenase [NAD(P)] activity
C	0005496	molecular_function	steroid binding
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006629	biological_process	lipid metabolic process
C	0006694	biological_process	steroid biosynthetic process
C	0006703	biological_process	estrogen biosynthetic process
C	0007040	biological_process	lysosome organization
C	0007519	biological_process	skeletal muscle tissue development
C	0008210	biological_process	estrogen metabolic process
C	0010467	biological_process	gene expression
C	0016491	molecular_function	oxidoreductase activity
C	0030283	molecular_function	testosterone dehydrogenase [NAD(P)] activity
C	0035410	molecular_function	dihydrotestosterone 17-beta-dehydrogenase activity
C	0036094	molecular_function	small molecule binding
C	0042803	molecular_function	protein homodimerization activity
C	0047035	molecular_function	testosterone dehydrogenase (NAD+) activity
C	0047045	molecular_function	testosterone 17-beta-dehydrogenase (NADP+) activity
C	0050661	molecular_function	NADP binding
C	0060348	biological_process	bone development
C	0060612	biological_process	adipose tissue development
C	0061370	biological_process	testosterone biosynthetic process
C	0070401	molecular_function	NADP+ binding
C	0071248	biological_process	cellular response to metal ion
C	0072582	molecular_function	17-beta-hydroxysteroid dehydrogenase (NADP+) activity
C	1903924	molecular_function	estradiol binding
D	0003824	molecular_function	catalytic activity
D	0004303	molecular_function	estradiol 17-beta-dehydrogenase [NAD(P)] activity
D	0005496	molecular_function	steroid binding
D	0005515	molecular_function	protein binding
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006629	biological_process	lipid metabolic process
D	0006694	biological_process	steroid biosynthetic process
D	0006703	biological_process	estrogen biosynthetic process
D	0007040	biological_process	lysosome organization
D	0007519	biological_process	skeletal muscle tissue development
D	0008210	biological_process	estrogen metabolic process
D	0010467	biological_process	gene expression
D	0016491	molecular_function	oxidoreductase activity
D	0030283	molecular_function	testosterone dehydrogenase [NAD(P)] activity
D	0035410	molecular_function	dihydrotestosterone 17-beta-dehydrogenase activity
D	0036094	molecular_function	small molecule binding
D	0042803	molecular_function	protein homodimerization activity
D	0047035	molecular_function	testosterone dehydrogenase (NAD+) activity
D	0047045	molecular_function	testosterone 17-beta-dehydrogenase (NADP+) activity
D	0050661	molecular_function	NADP binding
D	0060348	biological_process	bone development
D	0060612	biological_process	adipose tissue development
D	0061370	biological_process	testosterone biosynthetic process
D	0070401	molecular_function	NADP+ binding
D	0071248	biological_process	cellular response to metal ion
D	0072582	molecular_function	17-beta-hydroxysteroid dehydrogenase (NADP+) activity
D	1903924	molecular_function	estradiol binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 401

Chain	Residue
A	SER12
A	GLY13
A	HIS17
A	ARG44

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 B 400

Chain	Residue
C	HIS17
C	ARG44
C	THR190
C	PRO233
C	SER12
C	GLY13

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 C 403

Chain	Residue
A	SER11
A	ARG37
A	THR41
A	NAD361

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 D 402

Chain	Residue
C	SER11
C	ARG37
C	THR41
C	NAD363

site_id	AC5
Number of Residues	28
Details	BINDING SITE FOR RESIDUE NAD A 361

Chain	Residue
A	GLY9
A	CYS10
A	SER11
A	SER12
A	GLY13
A	ILE14
A	ARG37
A	LEU64
A	ASP65
A	VAL66
A	ARG67
A	ASN90
A	ALA91
A	GLY92
A	LEU93
A	VAL113
A	THR140
A	GLY141
A	TYR155
A	CYS185
A	GLY186
A	PRO187
A	VAL188
A	THR190
A	PHE192
A	LYS195
A	PHE226
C	SO4403

site_id	AC6
Number of Residues	20
Details	BINDING SITE FOR RESIDUE NAD B 364

Chain	Residue
B	GLY9
B	CYS10
B	SER11
B	SER12
B	ARG37
B	VAL66
B	ASN90
B	ALA91
B	GLY92
B	THR140
B	GLY141
B	SER142
B	TYR155
B	LYS159
B	CYS185
B	GLY186
B	PRO187
B	VAL188
B	THR190
B	PHE226

site_id	AC7
Number of Residues	30
Details	BINDING SITE FOR RESIDUE NAD C 363

Chain	Residue
C	GLY9
C	SER11
C	SER12
C	GLY13
C	ILE14
C	ARG37
C	LEU64
C	ASP65
C	VAL66
C	ASN90
C	ALA91
C	GLY92
C	LEU93
C	VAL113
C	THR140
C	GLY141
C	TYR155
C	LYS159
C	CYS185
C	GLY186
C	PRO187
C	VAL188
C	THR190
C	PHE192
C	PHE226
C	HOH512
C	HOH517
C	HOH529
C	HOH536
D	SO4402

site_id	AC8
Number of Residues	21
Details	BINDING SITE FOR RESIDUE NAD D 362

Chain	Residue
D	GLY9
D	SER11
D	SER12
D	ILE14
D	ARG37
D	LEU64
D	ASP65
D	VAL66
D	ASN90
D	GLY92
D	VAL113
D	THR140
D	GLY141
D	SER142
D	LYS159
D	CYS185
D	GLY186
D	PRO187
D	VAL188
D	THR190
D	HOH522

site_id	CAT
Number of Residues	3
Details	CATALYTIC SITE.

Chain	Residue
A	SER142
A	TYR155
A	LYS159

Functional Information from PROSITE/UniProt

site_id	PS00061
Number of Residues	29
Details	ADH_SHORT Short-chain dehydrogenases/reductases family signature. SvgglmglpfNdvYCASKFALeGLCeSLA

Chain	Residue	Details
A	SER142-ALA170

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Proton acceptor

Chain	Residue	Details
A	CYS156
B	CYS156
C	CYS156
D	CYS156

site_id	SWS_FT_FI2
Number of Residues	16
Details	BINDING: BINDING => ECO:0000269\|PubMed:8805577

Chain	Residue	Details
A	CYS10
C	VAL66
C	VAL143
C	PHE160
D	CYS10
D	VAL66
D	VAL143
D	PHE160
A	VAL66
A	VAL143
A	PHE160
B	CYS10
B	VAL66
B	VAL143
B	PHE160
C	CYS10

site_id	SWS_FT_FI3
Number of Residues	4
Details	MOD_RES: Phosphoserine; by PKA => ECO:0000269\|PubMed:8994190

Chain	Residue	Details
A	GLY135
B	GLY135
C	GLY135
D	GLY135

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)