1F73
CRYSTAL STRUCTURE ANALYSIS OF N-ACETYLNEURAMINATE LYASE FROM HAEMOPHILUS INFLUENZAE: CRYSTAL FORM III IN COMPLEX WITH SIALIC ACID ALDITOL
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0005975 | biological_process | carbohydrate metabolic process |
| A | 0008747 | molecular_function | N-acetylneuraminate lyase activity |
| A | 0016829 | molecular_function | lyase activity |
| A | 0019262 | biological_process | N-acetylneuraminate catabolic process |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0005975 | biological_process | carbohydrate metabolic process |
| B | 0008747 | molecular_function | N-acetylneuraminate lyase activity |
| B | 0016829 | molecular_function | lyase activity |
| B | 0019262 | biological_process | N-acetylneuraminate catabolic process |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0005975 | biological_process | carbohydrate metabolic process |
| C | 0008747 | molecular_function | N-acetylneuraminate lyase activity |
| C | 0016829 | molecular_function | lyase activity |
| C | 0019262 | biological_process | N-acetylneuraminate catabolic process |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005829 | cellular_component | cytosol |
| D | 0005975 | biological_process | carbohydrate metabolic process |
| D | 0008747 | molecular_function | N-acetylneuraminate lyase activity |
| D | 0016829 | molecular_function | lyase activity |
| D | 0019262 | biological_process | N-acetylneuraminate catabolic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 14 |
| Details | BINDING SITE FOR RESIDUE HMN A 700 |
| Chain | Residue |
| A | ALA10 |
| A | GLY206 |
| A | SER207 |
| A | LEU250 |
| A | HOH737 |
| A | HOH780 |
| A | TYR43 |
| A | GLY46 |
| A | SER47 |
| A | THR48 |
| A | LYS164 |
| A | GLY188 |
| A | ASP190 |
| A | GLU191 |
| site_id | AC2 |
| Number of Residues | 14 |
| Details | BINDING SITE FOR RESIDUE HMN B 701 |
| Chain | Residue |
| B | ALA10 |
| B | TYR43 |
| B | GLY46 |
| B | SER47 |
| B | THR48 |
| B | LYS164 |
| B | GLY188 |
| B | ASP190 |
| B | GLU191 |
| B | GLY206 |
| B | SER207 |
| B | LEU250 |
| B | HOH811 |
| B | HOH836 |
| site_id | AC3 |
| Number of Residues | 15 |
| Details | BINDING SITE FOR RESIDUE HMN C 702 |
| Chain | Residue |
| C | ALA10 |
| C | TYR43 |
| C | GLY46 |
| C | SER47 |
| C | THR48 |
| C | LYS164 |
| C | GLY188 |
| C | ASP190 |
| C | GLU191 |
| C | ILE205 |
| C | GLY206 |
| C | SER207 |
| C | LEU250 |
| C | HOH750 |
| C | HOH825 |
| site_id | AC4 |
| Number of Residues | 15 |
| Details | BINDING SITE FOR RESIDUE HMN D 703 |
| Chain | Residue |
| D | ALA10 |
| D | TYR43 |
| D | GLY46 |
| D | SER47 |
| D | THR48 |
| D | LYS164 |
| D | GLY188 |
| D | PHE189 |
| D | ASP190 |
| D | GLU191 |
| D | GLY206 |
| D | SER207 |
| D | LEU250 |
| D | HOH763 |
| D | HOH818 |
| site_id | AC5 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GOL B 704 |
| Chain | Residue |
| B | PHE52 |
| B | LEU54 |
| B | LYS59 |
| B | SER82 |
| B | GLU87 |
| B | HOH774 |
Functional Information from PROSITE/UniProt
| site_id | PS00217 |
| Number of Residues | 26 |
| Details | SUGAR_TRANSPORT_2 Sugar transport proteins signature 2. VdGLYvGGstgenfmLstEekkeifR |
| Chain | Residue | Details |
| A | VAL39-ARG64 |
| site_id | PS00228 |
| Number of Residues | 4 |
| Details | TUBULIN_B_AUTOREG Tubulin-beta mRNA autoregulation signal. MRDL |
| Chain | Residue | Details |
| A | MET1-LEU4 |
| site_id | PS00665 |
| Number of Residues | 18 |
| Details | DHDPS_1 Dihydrodipicolinate synthase signature 1. GLYvgGSTGENfmlsteE |
| Chain | Residue | Details |
| A | GLY41-GLU58 |
| site_id | PS00666 |
| Number of Residues | 31 |
| Details | DHDPS_2 Dihydrodipicolinate synthase signature 2. YSIPflTgvnMgieqfgelyknpk.VlGVKFT |
| Chain | Residue | Details |
| A | TYR136-THR166 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: Proton donor => ECO:0000255|HAMAP-Rule:MF_01237, ECO:0000305|PubMed:11031117 |
| Chain | Residue | Details |
| A | TYR136 | |
| B | TYR136 | |
| C | TYR136 | |
| D | TYR136 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | ACT_SITE: Schiff-base intermediate with substrate => ECO:0000255|HAMAP-Rule:MF_01237, ECO:0000305|PubMed:11031117 |
| Chain | Residue | Details |
| A | LYS164 | |
| B | LYS164 | |
| C | LYS164 | |
| D | LYS164 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 28 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_01237 |
| Chain | Residue | Details |
| A | SER47 | |
| B | THR166 | |
| B | GLY188 | |
| B | ASP190 | |
| B | GLU191 | |
| B | SER207 | |
| C | SER47 | |
| C | THR48 | |
| C | THR166 | |
| C | GLY188 | |
| C | ASP190 | |
| A | THR48 | |
| C | GLU191 | |
| C | SER207 | |
| D | SER47 | |
| D | THR48 | |
| D | THR166 | |
| D | GLY188 | |
| D | ASP190 | |
| D | GLU191 | |
| D | SER207 | |
| A | THR166 | |
| A | GLY188 | |
| A | ASP190 | |
| A | GLU191 | |
| A | SER207 | |
| B | SER47 | |
| B | THR48 |
