1F0Y
L-3-HYDROXYACYL-COA DEHYDROGENASE COMPLEXED WITH ACETOACETYL-COA AND NAD+
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003857 | molecular_function | 3-hydroxyacyl-CoA dehydrogenase (NAD+) activity |
| A | 0005654 | cellular_component | nucleoplasm |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0005759 | cellular_component | mitochondrial matrix |
| A | 0006629 | biological_process | lipid metabolic process |
| A | 0006631 | biological_process | fatty acid metabolic process |
| A | 0006635 | biological_process | fatty acid beta-oxidation |
| A | 0007283 | biological_process | spermatogenesis |
| A | 0009410 | biological_process | response to xenobiotic stimulus |
| A | 0009725 | biological_process | response to hormone |
| A | 0014823 | biological_process | response to activity |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| A | 0016740 | molecular_function | transferase activity |
| A | 0030154 | biological_process | cell differentiation |
| A | 0032868 | biological_process | response to insulin |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0046676 | biological_process | negative regulation of insulin secretion |
| A | 0050796 | biological_process | regulation of insulin secretion |
| A | 0070403 | molecular_function | NAD+ binding |
| A | 0120162 | biological_process | positive regulation of cold-induced thermogenesis |
| B | 0003857 | molecular_function | 3-hydroxyacyl-CoA dehydrogenase (NAD+) activity |
| B | 0005654 | cellular_component | nucleoplasm |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005739 | cellular_component | mitochondrion |
| B | 0005759 | cellular_component | mitochondrial matrix |
| B | 0006629 | biological_process | lipid metabolic process |
| B | 0006631 | biological_process | fatty acid metabolic process |
| B | 0006635 | biological_process | fatty acid beta-oxidation |
| B | 0007283 | biological_process | spermatogenesis |
| B | 0009410 | biological_process | response to xenobiotic stimulus |
| B | 0009725 | biological_process | response to hormone |
| B | 0014823 | biological_process | response to activity |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| B | 0016740 | molecular_function | transferase activity |
| B | 0030154 | biological_process | cell differentiation |
| B | 0032868 | biological_process | response to insulin |
| B | 0042802 | molecular_function | identical protein binding |
| B | 0046676 | biological_process | negative regulation of insulin secretion |
| B | 0050796 | biological_process | regulation of insulin secretion |
| B | 0070403 | molecular_function | NAD+ binding |
| B | 0120162 | biological_process | positive regulation of cold-induced thermogenesis |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 24 |
| Details | BINDING SITE FOR RESIDUE CAA A 351 |
| Chain | Residue |
| A | SER61 |
| A | VAL165 |
| A | ASN208 |
| A | LEU211 |
| A | PRO243 |
| A | MET244 |
| A | LEU249 |
| A | TYR252 |
| A | VAL253 |
| A | NAD350 |
| A | HOH801 |
| A | VAL65 |
| A | HOH1139 |
| A | HOH1186 |
| A | HOH1223 |
| B | GLY239 |
| B | ALA240 |
| A | LYS68 |
| A | SER137 |
| A | HIS158 |
| A | PHE160 |
| A | ASN161 |
| A | PRO162 |
| A | PRO164 |
| site_id | AC2 |
| Number of Residues | 22 |
| Details | BINDING SITE FOR RESIDUE CAA B 751 |
| Chain | Residue |
| A | GLY239 |
| B | SER61 |
| B | VAL65 |
| B | LYS68 |
| B | SER137 |
| B | HIS158 |
| B | PHE160 |
| B | ASN161 |
| B | PRO162 |
| B | PRO164 |
| B | VAL165 |
| B | ASN208 |
| B | LEU211 |
| B | PRO243 |
| B | MET244 |
| B | LEU249 |
| B | TYR252 |
| B | NAD750 |
| B | HOH804 |
| B | HOH1079 |
| B | HOH1220 |
| B | HOH1246 |
| site_id | AC3 |
| Number of Residues | 28 |
| Details | BINDING SITE FOR RESIDUE NAD A 350 |
| Chain | Residue |
| A | ILE21 |
| A | GLY24 |
| A | LEU25 |
| A | MET26 |
| A | ASP45 |
| A | GLN46 |
| A | ALA107 |
| A | ILE108 |
| A | GLU110 |
| A | LYS115 |
| A | ASN135 |
| A | SER137 |
| A | HIS158 |
| A | PHE159 |
| A | ASN161 |
| A | VAL253 |
| A | THR257 |
| A | LYS293 |
| A | CAA351 |
| A | HOH818 |
| A | HOH879 |
| A | HOH885 |
| A | HOH908 |
| A | HOH926 |
| A | HOH975 |
| A | HOH1066 |
| A | HOH1087 |
| A | HOH1181 |
| site_id | AC4 |
| Number of Residues | 25 |
| Details | BINDING SITE FOR RESIDUE NAD B 750 |
| Chain | Residue |
| B | GLY24 |
| B | LEU25 |
| B | MET26 |
| B | ASP45 |
| B | GLN46 |
| B | ALA107 |
| B | ILE108 |
| B | GLU110 |
| B | LYS115 |
| B | ASN135 |
| B | SER137 |
| B | HIS158 |
| B | PHE159 |
| B | ASN161 |
| B | VAL253 |
| B | THR257 |
| B | LYS293 |
| B | CAA751 |
| B | HOH815 |
| B | HOH831 |
| B | HOH864 |
| B | HOH927 |
| B | HOH944 |
| B | HOH1042 |
| B | HOH1222 |
Functional Information from PROSITE/UniProt
| site_id | PS00067 |
| Number of Residues | 25 |
| Details | 3HCDH 3-hydroxyacyl-CoA dehydrogenase signature. DtpGFIvNRllvPYLmeair.LYerG |
| Chain | Residue | Details |
| A | ASP201-GLY225 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 14 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:10231530, ECO:0000269|PubMed:10840044 |
| Chain | Residue | Details |
| A | ALA28 | |
| B | ASN116 | |
| B | ARG121 | |
| B | SER143 | |
| B | LYS167 | |
| B | TYR299 | |
| A | LEU51 | |
| A | ASN116 | |
| A | ARG121 | |
| A | SER143 | |
| A | LYS167 | |
| A | TYR299 | |
| B | ALA28 | |
| B | LEU51 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:10840044 |
| Chain | Residue | Details |
| A | LYS67 | |
| A | PRO74 | |
| B | LYS67 | |
| B | PRO74 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | SITE: Important for catalytic activity => ECO:0000305 |
| Chain | Residue | Details |
| A | PRO164 | |
| B | PRO164 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | MOD_RES: N6-succinyllysine => ECO:0000250|UniProtKB:Q61425 |
| Chain | Residue | Details |
| A | PRO74 | |
| A | LYS200 | |
| B | PRO74 | |
| B | LYS200 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 16 |
| Details | MOD_RES: N6-succinyllysine; alternate => ECO:0000250|UniProtKB:Q61425 |
| Chain | Residue | Details |
| A | LYS75 | |
| B | VAL81 | |
| B | THR130 | |
| B | GLN179 | |
| B | VAL186 | |
| B | PRO196 | |
| B | ILE206 | |
| B | ALA235 | |
| A | VAL81 | |
| A | THR130 | |
| A | GLN179 | |
| A | VAL186 | |
| A | PRO196 | |
| A | ILE206 | |
| A | ALA235 | |
| B | LYS75 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 4 |
| Details | MOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:Q61425 |
| Chain | Residue | Details |
| A | PHE119 | |
| A | LYS173 | |
| B | PHE119 | |
| B | LYS173 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 2 |
| Details | MOD_RES: N6-(2-hydroxyisobutyryl)lysine => ECO:0000269|PubMed:29192674 |
| Chain | Residue | Details |
| A | ARG121 | |
| B | ARG121 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 2hdh |
| Chain | Residue | Details |
| A | ASN208 | |
| A | SER137 | |
| A | HIS158 |
| site_id | CSA2 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 2hdh |
| Chain | Residue | Details |
| B | ASN208 | |
| B | SER137 | |
| B | HIS158 |
| site_id | MCSA1 |
| Number of Residues | 4 |
| Details | M-CSA 336 |
| Chain | Residue | Details |
| A | SER137 | electrostatic stabiliser, hydrogen bond donor |
| A | HIS158 | proton acceptor, proton donor |
| A | GLU170 | electrostatic stabiliser, hydrogen bond acceptor, increase basicity |
| A | ASN208 | electrostatic stabiliser, hydrogen bond donor |
| site_id | MCSA2 |
| Number of Residues | 4 |
| Details | M-CSA 336 |
| Chain | Residue | Details |
| B | SER137 | electrostatic stabiliser, hydrogen bond donor |
| B | HIS158 | proton acceptor, proton donor |
| B | GLU170 | electrostatic stabiliser, hydrogen bond acceptor, increase basicity |
| B | ASN208 | electrostatic stabiliser, hydrogen bond donor |
