1F0Y
L-3-HYDROXYACYL-COA DEHYDROGENASE COMPLEXED WITH ACETOACETYL-COA AND NAD+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-08-27 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.488, 87.971, 159.002 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.21 * |
| Rwork | 0.210 |
| R-free | 0.23900 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.600 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | CrystalClear ((MSC/RIGAKU)) |
| Phasing software | CNS (0.3) |
| Refinement software | CNS (0.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.062 | 0.176 |
| Number of reflections | 66610 | |
| <I/σ(I)> | 14.9 | |
| Completeness [%] | 91.6 | 54.4 |
| Redundancy | 3.39 | 1.65 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | Barycki, J.J., (1999) Biochemistry, 38, 5786. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | NAD+ | 5 (mM) | |
| 3 | 1 | reservoir | N-[2-acetamido]-2-iminodiacetic acid | 50 (mM) | |
| 4 | 1 | reservoir | PEG4000 | 14-19 (%) |
