1EGN
CELLOBIOHYDROLASE CEL7A (E223S, A224H, L225V, T226A, D262G) MUTANT
Functional Information from GO Data
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | ACT_SITE: Nucleophile => ECO:0000269|PubMed:24341799, ECO:0000305|PubMed:8036495 |
| Chain | Residue | Details |
| A | GLU212 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | ACT_SITE: Proton donor/acceptor => ECO:0000269|PubMed:24341799, ECO:0000305|PubMed:8036495 |
| Chain | Residue | Details |
| A | GLU217 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | SITE: Not glycosylated => ECO:0000269|PubMed:9746354 |
| Chain | Residue | Details |
| A | ASN64 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | MOD_RES: Pyrrolidone carboxylic acid => ECO:0000269|DOI:10.1016/0014-5793(80)81006-4, ECO:0000269|DOI:10.1038/nbt1083-687, ECO:0007744|PDB:1EGN, ECO:0007744|PDB:1Q2B, ECO:0007744|PDB:1Q2E |
| Chain | Residue | Details |
| A | PCA1 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | CARBOHYD: N-linked (GlcNAc) asparagine => ECO:0000269|PubMed:9746354 |
| Chain | Residue | Details |
| A | ASN45 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 1 |
| Details | CARBOHYD: N-linked (GlcNAc) asparagine => ECO:0000269|PubMed:24341799, ECO:0000269|PubMed:8036495, ECO:0000269|PubMed:9466911, ECO:0000269|PubMed:9746354 |
| Chain | Residue | Details |
| A | ASN270 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 1 |
| Details | CARBOHYD: N-linked (GlcNAc) asparagine => ECO:0000269|PubMed:24341799, ECO:0000269|PubMed:9466911, ECO:0000269|PubMed:9746354 |
| Chain | Residue | Details |
| A | ASN384 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1cel |
| Chain | Residue | Details |
| A | GLU212 | |
| A | HIS228 | |
| A | ASP214 | |
| A | GLU217 |
| site_id | MCSA1 |
| Number of Residues | 4 |
| Details | M-CSA 444 |
| Chain | Residue | Details |
| A | GLU212 | covalent catalysis |
| A | ASP214 | modifies pKa |
| A | GLU217 | proton shuttle (general acid/base) |
| A | HIS228 | modifies pKa |
