1EGN
CELLOBIOHYDROLASE CEL7A (E223S, A224H, L225V, T226A, D262G) MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-05-30 |
| Detector | MARRESEARCH |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 83.110, 83.340, 110.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| R-factor | 0.24 |
| Rwork | 0.240 |
| R-free | 0.27000 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.064 | 0.237 |
| Number of reflections | 50924 * | |
| <I/σ(I)> | 24.6 | |
| Completeness [%] | 100.0 * | 99.9 |
| Redundancy | 4.7 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | sodium acetate, PEG 5000 monomethyl-ether, glycerol, sodium morpholine-ethane-sulphonic acid, cobalt chloride , pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 1.2 (mg/ml) | |
| 2 | 1 | drop | sodium acetate | 10 (mM) | |
| 3 | 1 | reservoir | PEG5000 MME | 18.75 (%) | |
| 4 | 1 | reservoir | glycerol | 12.5 (%) | |
| 5 | 1 | reservoir | sodium MES | 20 (mM) | |
| 6 | 1 | reservoir | 20 (mM) |
