1EGD

STRUCTURE OF T255E, E376G MUTANT OF HUMAN MEDIUM CHAIN ACYL-COA DEHYDROGENASE

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001889	biological_process	liver development
A	0003995	molecular_function	acyl-CoA dehydrogenase activity
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005759	cellular_component	mitochondrial matrix
A	0005978	biological_process	glycogen biosynthetic process
A	0006082	biological_process	organic acid metabolic process
A	0006111	biological_process	regulation of gluconeogenesis
A	0006629	biological_process	lipid metabolic process
A	0006631	biological_process	fatty acid metabolic process
A	0006635	biological_process	fatty acid beta-oxidation
A	0007507	biological_process	heart development
A	0009409	biological_process	response to cold
A	0009437	biological_process	carnitine metabolic process
A	0009791	biological_process	post-embryonic development
A	0016491	molecular_function	oxidoreductase activity
A	0016627	molecular_function	oxidoreductase activity, acting on the CH-CH group of donors
A	0019254	biological_process	carnitine metabolic process, CoA-linked
A	0030424	cellular_component	axon
A	0031966	cellular_component	mitochondrial membrane
A	0033539	biological_process	fatty acid beta-oxidation using acyl-CoA dehydrogenase
A	0042594	biological_process	response to starvation
A	0042802	molecular_function	identical protein binding
A	0045329	biological_process	carnitine biosynthetic process
A	0050660	molecular_function	flavin adenine dinucleotide binding
A	0051791	biological_process	medium-chain fatty acid metabolic process
A	0051793	biological_process	medium-chain fatty acid catabolic process
A	0055007	biological_process	cardiac muscle cell differentiation
A	0070991	molecular_function	medium-chain fatty acyl-CoA dehydrogenase activity
B	0001889	biological_process	liver development
B	0003995	molecular_function	acyl-CoA dehydrogenase activity
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005759	cellular_component	mitochondrial matrix
B	0005978	biological_process	glycogen biosynthetic process
B	0006082	biological_process	organic acid metabolic process
B	0006111	biological_process	regulation of gluconeogenesis
B	0006629	biological_process	lipid metabolic process
B	0006631	biological_process	fatty acid metabolic process
B	0006635	biological_process	fatty acid beta-oxidation
B	0007507	biological_process	heart development
B	0009409	biological_process	response to cold
B	0009437	biological_process	carnitine metabolic process
B	0009791	biological_process	post-embryonic development
B	0016491	molecular_function	oxidoreductase activity
B	0016627	molecular_function	oxidoreductase activity, acting on the CH-CH group of donors
B	0019254	biological_process	carnitine metabolic process, CoA-linked
B	0030424	cellular_component	axon
B	0031966	cellular_component	mitochondrial membrane
B	0033539	biological_process	fatty acid beta-oxidation using acyl-CoA dehydrogenase
B	0042594	biological_process	response to starvation
B	0042802	molecular_function	identical protein binding
B	0045329	biological_process	carnitine biosynthetic process
B	0050660	molecular_function	flavin adenine dinucleotide binding
B	0051791	biological_process	medium-chain fatty acid metabolic process
B	0051793	biological_process	medium-chain fatty acid catabolic process
B	0055007	biological_process	cardiac muscle cell differentiation
B	0070991	molecular_function	medium-chain fatty acyl-CoA dehydrogenase activity
C	0001889	biological_process	liver development
C	0003995	molecular_function	acyl-CoA dehydrogenase activity
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0005739	cellular_component	mitochondrion
C	0005759	cellular_component	mitochondrial matrix
C	0005978	biological_process	glycogen biosynthetic process
C	0006082	biological_process	organic acid metabolic process
C	0006111	biological_process	regulation of gluconeogenesis
C	0006629	biological_process	lipid metabolic process
C	0006631	biological_process	fatty acid metabolic process
C	0006635	biological_process	fatty acid beta-oxidation
C	0007507	biological_process	heart development
C	0009409	biological_process	response to cold
C	0009437	biological_process	carnitine metabolic process
C	0009791	biological_process	post-embryonic development
C	0016491	molecular_function	oxidoreductase activity
C	0016627	molecular_function	oxidoreductase activity, acting on the CH-CH group of donors
C	0019254	biological_process	carnitine metabolic process, CoA-linked
C	0030424	cellular_component	axon
C	0031966	cellular_component	mitochondrial membrane
C	0033539	biological_process	fatty acid beta-oxidation using acyl-CoA dehydrogenase
C	0042594	biological_process	response to starvation
C	0042802	molecular_function	identical protein binding
C	0045329	biological_process	carnitine biosynthetic process
C	0050660	molecular_function	flavin adenine dinucleotide binding
C	0051791	biological_process	medium-chain fatty acid metabolic process
C	0051793	biological_process	medium-chain fatty acid catabolic process
C	0055007	biological_process	cardiac muscle cell differentiation
C	0070991	molecular_function	medium-chain fatty acyl-CoA dehydrogenase activity
D	0001889	biological_process	liver development
D	0003995	molecular_function	acyl-CoA dehydrogenase activity
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005739	cellular_component	mitochondrion
D	0005759	cellular_component	mitochondrial matrix
D	0005978	biological_process	glycogen biosynthetic process
D	0006082	biological_process	organic acid metabolic process
D	0006111	biological_process	regulation of gluconeogenesis
D	0006629	biological_process	lipid metabolic process
D	0006631	biological_process	fatty acid metabolic process
D	0006635	biological_process	fatty acid beta-oxidation
D	0007507	biological_process	heart development
D	0009409	biological_process	response to cold
D	0009437	biological_process	carnitine metabolic process
D	0009791	biological_process	post-embryonic development
D	0016491	molecular_function	oxidoreductase activity
D	0016627	molecular_function	oxidoreductase activity, acting on the CH-CH group of donors
D	0019254	biological_process	carnitine metabolic process, CoA-linked
D	0030424	cellular_component	axon
D	0031966	cellular_component	mitochondrial membrane
D	0033539	biological_process	fatty acid beta-oxidation using acyl-CoA dehydrogenase
D	0042594	biological_process	response to starvation
D	0042802	molecular_function	identical protein binding
D	0045329	biological_process	carnitine biosynthetic process
D	0050660	molecular_function	flavin adenine dinucleotide binding
D	0051791	biological_process	medium-chain fatty acid metabolic process
D	0051793	biological_process	medium-chain fatty acid catabolic process
D	0055007	biological_process	cardiac muscle cell differentiation
D	0070991	molecular_function	medium-chain fatty acyl-CoA dehydrogenase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	23
Details	BINDING SITE FOR RESIDUE FAD A 399

Chain	Residue
A	TRP166
A	THR168
A	ASN214
A	ILE371
A	THR378
A	GLN380
A	HOH2012
A	HOH2075
A	HOH2078
B	ARG281
B	THR283
B	PHE284
B	LEU288
B	ILE294
B	GLN349
B	ILE350
B	GLY353
D	GLN292
A	TYR133
A	VAL135
A	THR136
A	GLY141
A	SER142

---

site_id	AC2
Number of Residues	25
Details	BINDING SITE FOR RESIDUE FAD B 399

Chain	Residue
A	ARG281
A	THR283
A	PHE284
A	LEU288
A	ILE294
A	GLN349
A	ILE350
A	GLY353
B	TYR133
B	VAL135
B	THR136
B	GLY141
B	SER142
B	TRP166
B	THR168
B	ASN214
B	ILE374
B	TYR375
B	THR378
B	GLN380
B	HOH2008
B	HOH2048
B	HOH2056
B	HOH2076
C	GLN292

---

site_id	AC3
Number of Residues	20
Details	BINDING SITE FOR RESIDUE FAD C 399

Chain	Residue
B	GLN292
C	TYR133
C	VAL135
C	THR136
C	GLY141
C	SER142
C	TRP166
C	THR168
C	ILE374
C	THR378
C	GLN380
C	HOH2095
D	ARG281
D	THR283
D	PHE284
D	ILE294
D	GLN349
D	ILE350
D	GLY353
D	HOH2004

---

site_id	AC4
Number of Residues	23
Details	BINDING SITE FOR RESIDUE FAD D 399

Chain	Residue
A	GLN292
C	ARG281
C	THR283
C	PHE284
C	LEU288
C	HIS291
C	ILE294
C	GLN349
C	ILE350
C	GLY353
C	HOH2222
D	TYR133
D	VAL135
D	THR136
D	GLY141
D	SER142
D	TRP166
D	THR168
D	ASN214
D	THR222
D	THR378
D	GLN380
D	HOH2025

---

site_id	CA1
Number of Residues	1
Details	CATALYTIC BASE IN CHAIN A

Chain	Residue
A	GLY376

---

site_id	CA2
Number of Residues	1
Details	CATALYTIC BASE IN CHAIN B

Chain	Residue
B	GLY376

---

site_id	CA3
Number of Residues	1
Details	CATALYTIC BASE IN CHAIN C

Chain	Residue
C	GLY376

---

site_id	CA4
Number of Residues	1
Details	CATALYTIC BASE IN CHAIN D

Chain	Residue
D	GLY376

---

Functional Information from PROSITE/UniProt

site_id	PS00072
Number of Residues	13
Details	ACYL_COA_DH_1 Acyl-CoA dehydrogenases signature 1. CVTEpgAGSDvaG

Chain	Residue	Details
A	CYS134-GLY146

---

site_id	PS00073
Number of Residues	20
Details	ACYL_COA_DH_2 Acyl-CoA dehydrogenases signature 2. QiLGGnGFntEypveKlmrD

Chain	Residue	Details
A	GLN349-ASP368

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Proton acceptor => ECO:0000269|PubMed:1970566, ECO:0000269|PubMed:8823176

Chain	Residue	Details
A	GLY376
B	GLY376
C	GLY376
D	GLY376

---

site_id	SWS_FT_FI2
Number of Residues	12
Details	BINDING: in other chain => ECO:0000269|PubMed:15159392, ECO:0000269|PubMed:15975918, ECO:0000269|PubMed:8823176, ECO:0000269|Ref.23, ECO:0007744|PDB:1EGC, ECO:0007744|PDB:1EGD, ECO:0007744|PDB:1EGE, ECO:0007744|PDB:1T9G, ECO:0007744|PDB:2A1T, ECO:0007744|PDB:4P13

Chain	Residue	Details
A	TYR133
D	TYR133
D	TRP166
D	HIS291
A	TRP166
A	HIS291
B	TYR133
B	TRP166
B	HIS291
C	TYR133
C	TRP166
C	HIS291

---

site_id	SWS_FT_FI3
Number of Residues	16
Details	BINDING: BINDING => ECO:0000269|PubMed:8823176, ECO:0007744|PDB:1EGC

Chain	Residue	Details
A	SER142
C	ASP253
C	ARG256
C	GLY376
D	SER142
D	ASP253
D	ARG256
D	GLY376
A	ASP253
A	ARG256
A	GLY376
B	SER142
B	ASP253
B	ARG256
B	GLY376
C	SER142

---

site_id	SWS_FT_FI4
Number of Residues	8
Details	BINDING: BINDING => ECO:0000269|PubMed:15159392, ECO:0000269|PubMed:15975918, ECO:0000269|PubMed:8823176, ECO:0000269|Ref.23, ECO:0007744|PDB:1EGC, ECO:0007744|PDB:1EGD, ECO:0007744|PDB:1EGE, ECO:0007744|PDB:1T9G, ECO:0007744|PDB:2A1T, ECO:0007744|PDB:4P13

Chain	Residue	Details
A	ARG281
A	GLN349
B	ARG281
B	GLN349
C	ARG281
C	GLN349
D	ARG281
D	GLN349

---

site_id	SWS_FT_FI5
Number of Residues	20
Details	MOD_RES: N6-succinyllysine; alternate => ECO:0000250|UniProtKB:P45952

Chain	Residue	Details
A	LYS44
B	LYS246
C	LYS44
C	LYS187
C	LYS192
C	LYS234
C	LYS246
D	LYS44
D	LYS187
D	LYS192
D	LYS234
A	LYS187
D	LYS246
A	LYS192
A	LYS234
A	LYS246
B	LYS44
B	LYS187
B	LYS192
B	LYS234

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	MOD_RES: N6-succinyllysine => ECO:0000250|UniProtKB:P45952

Chain	Residue	Details
A	LYS154
B	LYS154
C	LYS154
D	LYS154

---

site_id	SWS_FT_FI7
Number of Residues	8
Details	MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861

Chain	Residue	Details
A	LYS254
A	LYS276
B	LYS254
B	LYS276
C	LYS254
C	LYS276
D	LYS254
D	LYS276

---

site_id	SWS_FT_FI8
Number of Residues	4
Details	MOD_RES: Phosphothreonine => ECO:0000250|UniProtKB:P45952

Chain	Residue	Details
A	THR326
B	THR326
C	THR326
D	THR326

---