1EGD

STRUCTURE OF T255E, E376G MUTANT OF HUMAN MEDIUM CHAIN ACYL-COA DEHYDROGENASE

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1993-06-18
Detector	RIGAKU
Spacegroup name	P 43 21 2
Unit cell lengths	170.180, 170.180, 150.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.400
R-factor	0.208
Rwork	0.208
RMSD bond length	0.009
RMSD bond angle	22.500 *
Data reduction software	R-AXIS (IIC)
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.400 *
Rmerge	0.073 *
Total number of observations	204212 *
Number of reflections	66928
Completeness [%]	77.7
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme		0.027mg
2	1	drop	Tris acetate	140 (mM)
3	1	drop	PEG4000	8.0 (%(w/v))
4	1	reservoir	PEG4000	50 (%)