Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

1EGC

STRUCTURE OF T255E, E376G MUTANT OF HUMAN MEDIUM CHAIN ACYL-COA DEHYDROGENASE COMPLEXED WITH OCTANOYL-COA

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001889	biological_process	liver development
A	0003995	molecular_function	acyl-CoA dehydrogenase activity
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005759	cellular_component	mitochondrial matrix
A	0005978	biological_process	glycogen biosynthetic process
A	0006082	biological_process	organic acid metabolic process
A	0006111	biological_process	regulation of gluconeogenesis
A	0006631	biological_process	fatty acid metabolic process
A	0006635	biological_process	fatty acid beta-oxidation
A	0007507	biological_process	heart development
A	0009409	biological_process	response to cold
A	0009437	biological_process	carnitine metabolic process
A	0009791	biological_process	post-embryonic development
A	0016491	molecular_function	oxidoreductase activity
A	0016627	molecular_function	oxidoreductase activity, acting on the CH-CH group of donors
A	0019254	biological_process	carnitine metabolic process, CoA-linked
A	0030424	cellular_component	axon
A	0031966	cellular_component	mitochondrial membrane
A	0033539	biological_process	fatty acid beta-oxidation using acyl-CoA dehydrogenase
A	0042594	biological_process	response to starvation
A	0042802	molecular_function	identical protein binding
A	0045329	biological_process	carnitine biosynthetic process
A	0050660	molecular_function	flavin adenine dinucleotide binding
A	0051791	biological_process	medium-chain fatty acid metabolic process
A	0051793	biological_process	medium-chain fatty acid catabolic process
A	0055007	biological_process	cardiac muscle cell differentiation
A	0070991	molecular_function	medium-chain fatty acyl-CoA dehydrogenase activity
B	0001889	biological_process	liver development
B	0003995	molecular_function	acyl-CoA dehydrogenase activity
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005759	cellular_component	mitochondrial matrix
B	0005978	biological_process	glycogen biosynthetic process
B	0006082	biological_process	organic acid metabolic process
B	0006111	biological_process	regulation of gluconeogenesis
B	0006631	biological_process	fatty acid metabolic process
B	0006635	biological_process	fatty acid beta-oxidation
B	0007507	biological_process	heart development
B	0009409	biological_process	response to cold
B	0009437	biological_process	carnitine metabolic process
B	0009791	biological_process	post-embryonic development
B	0016491	molecular_function	oxidoreductase activity
B	0016627	molecular_function	oxidoreductase activity, acting on the CH-CH group of donors
B	0019254	biological_process	carnitine metabolic process, CoA-linked
B	0030424	cellular_component	axon
B	0031966	cellular_component	mitochondrial membrane
B	0033539	biological_process	fatty acid beta-oxidation using acyl-CoA dehydrogenase
B	0042594	biological_process	response to starvation
B	0042802	molecular_function	identical protein binding
B	0045329	biological_process	carnitine biosynthetic process
B	0050660	molecular_function	flavin adenine dinucleotide binding
B	0051791	biological_process	medium-chain fatty acid metabolic process
B	0051793	biological_process	medium-chain fatty acid catabolic process
B	0055007	biological_process	cardiac muscle cell differentiation
B	0070991	molecular_function	medium-chain fatty acyl-CoA dehydrogenase activity
C	0001889	biological_process	liver development
C	0003995	molecular_function	acyl-CoA dehydrogenase activity
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0005739	cellular_component	mitochondrion
C	0005759	cellular_component	mitochondrial matrix
C	0005978	biological_process	glycogen biosynthetic process
C	0006082	biological_process	organic acid metabolic process
C	0006111	biological_process	regulation of gluconeogenesis
C	0006631	biological_process	fatty acid metabolic process
C	0006635	biological_process	fatty acid beta-oxidation
C	0007507	biological_process	heart development
C	0009409	biological_process	response to cold
C	0009437	biological_process	carnitine metabolic process
C	0009791	biological_process	post-embryonic development
C	0016491	molecular_function	oxidoreductase activity
C	0016627	molecular_function	oxidoreductase activity, acting on the CH-CH group of donors
C	0019254	biological_process	carnitine metabolic process, CoA-linked
C	0030424	cellular_component	axon
C	0031966	cellular_component	mitochondrial membrane
C	0033539	biological_process	fatty acid beta-oxidation using acyl-CoA dehydrogenase
C	0042594	biological_process	response to starvation
C	0042802	molecular_function	identical protein binding
C	0045329	biological_process	carnitine biosynthetic process
C	0050660	molecular_function	flavin adenine dinucleotide binding
C	0051791	biological_process	medium-chain fatty acid metabolic process
C	0051793	biological_process	medium-chain fatty acid catabolic process
C	0055007	biological_process	cardiac muscle cell differentiation
C	0070991	molecular_function	medium-chain fatty acyl-CoA dehydrogenase activity
D	0001889	biological_process	liver development
D	0003995	molecular_function	acyl-CoA dehydrogenase activity
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005739	cellular_component	mitochondrion
D	0005759	cellular_component	mitochondrial matrix
D	0005978	biological_process	glycogen biosynthetic process
D	0006082	biological_process	organic acid metabolic process
D	0006111	biological_process	regulation of gluconeogenesis
D	0006631	biological_process	fatty acid metabolic process
D	0006635	biological_process	fatty acid beta-oxidation
D	0007507	biological_process	heart development
D	0009409	biological_process	response to cold
D	0009437	biological_process	carnitine metabolic process
D	0009791	biological_process	post-embryonic development
D	0016491	molecular_function	oxidoreductase activity
D	0016627	molecular_function	oxidoreductase activity, acting on the CH-CH group of donors
D	0019254	biological_process	carnitine metabolic process, CoA-linked
D	0030424	cellular_component	axon
D	0031966	cellular_component	mitochondrial membrane
D	0033539	biological_process	fatty acid beta-oxidation using acyl-CoA dehydrogenase
D	0042594	biological_process	response to starvation
D	0042802	molecular_function	identical protein binding
D	0045329	biological_process	carnitine biosynthetic process
D	0050660	molecular_function	flavin adenine dinucleotide binding
D	0051791	biological_process	medium-chain fatty acid metabolic process
D	0051793	biological_process	medium-chain fatty acid catabolic process
D	0055007	biological_process	cardiac muscle cell differentiation
D	0070991	molecular_function	medium-chain fatty acyl-CoA dehydrogenase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	20
Details	BINDING SITE FOR RESIDUE CO8 A 400

Chain	Residue
A	THR168
A	ASN191
A	PHE245
A	MET249
A	PHE252
A	ASP253
A	ARG256
A	THR326
A	TYR375
A	GLY376
A	GLY377
A	ARG388
A	FAD399
A	HOH2034
B	PHE284
A	GLU99
A	LEU103
A	GLY141
A	SER142
A	VAL144

site_id	AC2
Number of Residues	23
Details	BINDING SITE FOR RESIDUE CO8 B 400

Chain	Residue
A	PHE284
B	GLU99
B	LEU103
B	THR136
B	GLY141
B	SER142
B	VAL144
B	ALA145
B	ASN191
B	PHE245
B	MET249
B	PHE252
B	ASP253
B	ARG256
B	VAL259
B	THR326
B	TYR375
B	GLY376
B	GLY377
B	ILE381
B	FAD399
C	ASN191
C	LYS192

site_id	AC3
Number of Residues	20
Details	BINDING SITE FOR RESIDUE CO8 C 400

Chain	Residue
B	ASN191
B	LYS192
C	GLU99
C	GLY100
C	LEU103
C	THR136
C	SER142
C	VAL144
C	ASN191
C	PHE245
C	MET249
C	PHE252
C	ASP253
C	VAL259
C	THR326
C	TYR375
C	GLY376
C	GLY377
C	ARG388
C	FAD399

site_id	AC4
Number of Residues	21
Details	BINDING SITE FOR RESIDUE CO8 D 400

Chain	Residue
D	GLN95
D	THR96
D	LEU103
D	GLY141
D	SER142
D	VAL144
D	ALA145
D	ASN191
D	PHE245
D	MET249
D	PHE252
D	ASP253
D	ARG256
D	ARG324
D	THR326
D	TYR375
D	GLY376
D	GLY377
D	ILE381
D	ARG388
D	FAD399

site_id	AC5
Number of Residues	24
Details	BINDING SITE FOR RESIDUE FAD A 399

Chain	Residue
A	THR378
A	GLN380
A	CO8400
B	TYR277
B	ARG281
B	THR283
B	LEU288
B	HIS291
B	ILE294
B	GLN349
B	ILE350
B	GLY353
D	GLN292
A	TYR133
A	VAL135
A	THR136
A	GLY141
A	SER142
A	TRP166
A	THR168
A	THR222
A	ILE371
A	ILE374
A	TYR375

site_id	AC6
Number of Residues	24
Details	BINDING SITE FOR RESIDUE FAD B 399

Chain	Residue
A	ARG281
A	THR283
A	PHE284
A	LEU288
A	HIS291
A	ILE294
A	GLN349
A	ILE350
A	GLY353
B	TYR133
B	VAL135
B	THR136
B	GLY141
B	SER142
B	TRP166
B	THR168
B	THR222
B	ILE374
B	TYR375
B	THR378
B	GLN380
B	CO8400
B	HOH2050
C	GLN292

site_id	AC7
Number of Residues	25
Details	BINDING SITE FOR RESIDUE FAD C 399

Chain	Residue
B	GLN292
C	TYR133
C	VAL135
C	THR136
C	GLY141
C	SER142
C	TRP166
C	ILE167
C	THR168
C	ASN214
C	ILE374
C	TYR375
C	THR378
C	GLN380
C	CO8400
C	HOH2010
D	ARG281
D	THR283
D	LEU288
D	HIS291
D	ILE294
D	GLN349
D	ILE350
D	GLY352
D	GLY353

site_id	AC8
Number of Residues	24
Details	BINDING SITE FOR RESIDUE FAD D 399

Chain	Residue
A	GLN292
C	ARG281
C	THR283
C	PHE284
C	LEU288
C	HIS291
C	ILE294
C	GLN349
C	ILE350
C	GLY353
D	TYR133
D	VAL135
D	THR136
D	GLY141
D	SER142
D	TRP166
D	THR168
D	THR222
D	ILE374
D	TYR375
D	THR378
D	GLN380
D	CO8400
D	HOH2119

site_id	CA1
Number of Residues	1
Details	CATALYTIC BASE IN CHAIN A

Chain	Residue
A	GLU255

site_id	CA2
Number of Residues	1
Details	CATALYTIC BASE IN CHAIN B

Chain	Residue
B	GLU255

site_id	CA3
Number of Residues	1
Details	CATALYTIC BASE IN CHAIN C

Chain	Residue
C	GLU255

site_id	CA4
Number of Residues	1
Details	CATALYTIC BASE IN CHAIN D

Chain	Residue
D	GLU255

Functional Information from PROSITE/UniProt

site_id	PS00072
Number of Residues	13
Details	ACYL_COA_DH_1 Acyl-CoA dehydrogenases signature 1. CVTEpgAGSDvaG

Chain	Residue	Details
A	CYS134-GLY146

site_id	PS00073
Number of Residues	20
Details	ACYL_COA_DH_2 Acyl-CoA dehydrogenases signature 2. QiLGGnGFntEypveKlmrD

Chain	Residue	Details
A	GLN349-ASP368

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Proton acceptor => ECO:0000269\|PubMed:1970566, ECO:0000269\|PubMed:8823176

Chain	Residue	Details
A	GLY376
B	GLY376
C	GLY376
D	GLY376

site_id	SWS_FT_FI2
Number of Residues	12
Details	BINDING: in other chain => ECO:0000269\|PubMed:15159392, ECO:0000269\|PubMed:15975918, ECO:0000269\|PubMed:8823176, ECO:0000269\|Ref.23, ECO:0007744\|PDB:1EGC, ECO:0007744\|PDB:1EGD, ECO:0007744\|PDB:1EGE, ECO:0007744\|PDB:1T9G, ECO:0007744\|PDB:2A1T, ECO:0007744\|PDB:4P13

Chain	Residue	Details
A	TYR133
D	TYR133
D	TRP166
D	HIS291
A	TRP166
A	HIS291
B	TYR133
B	TRP166
B	HIS291
C	TYR133
C	TRP166
C	HIS291

site_id	SWS_FT_FI3
Number of Residues	16
Details	BINDING: BINDING => ECO:0000269\|PubMed:8823176, ECO:0007744\|PDB:1EGC

Chain	Residue	Details
A	SER142
C	ASP253
C	ARG256
C	GLY376
D	SER142
D	ASP253
D	ARG256
D	GLY376
A	ASP253
A	ARG256
A	GLY376
B	SER142
B	ASP253
B	ARG256
B	GLY376
C	SER142

site_id	SWS_FT_FI4
Number of Residues	8
Details	BINDING: BINDING => ECO:0000269\|PubMed:15159392, ECO:0000269\|PubMed:15975918, ECO:0000269\|PubMed:8823176, ECO:0000269\|Ref.23, ECO:0007744\|PDB:1EGC, ECO:0007744\|PDB:1EGD, ECO:0007744\|PDB:1EGE, ECO:0007744\|PDB:1T9G, ECO:0007744\|PDB:2A1T, ECO:0007744\|PDB:4P13

Chain	Residue	Details
A	ARG281
A	GLN349
B	ARG281
B	GLN349
C	ARG281
C	GLN349
D	ARG281
D	GLN349

site_id	SWS_FT_FI5
Number of Residues	20
Details	MOD_RES: N6-succinyllysine; alternate => ECO:0000250\|UniProtKB:P45952

Chain	Residue	Details
A	LYS44
B	LYS246
C	LYS44
C	LYS187
C	LYS192
C	LYS234
C	LYS246
D	LYS44
D	LYS187
D	LYS192
D	LYS234
A	LYS187
D	LYS246
A	LYS192
A	LYS234
A	LYS246
B	LYS44
B	LYS187
B	LYS192
B	LYS234

site_id	SWS_FT_FI6
Number of Residues	4
Details	MOD_RES: N6-succinyllysine => ECO:0000250\|UniProtKB:P45952

Chain	Residue	Details
A	LYS154
B	LYS154
C	LYS154
D	LYS154

site_id	SWS_FT_FI7
Number of Residues	8
Details	MOD_RES: N6-acetyllysine => ECO:0007744\|PubMed:19608861

Chain	Residue	Details
A	LYS254
A	LYS276
B	LYS254
B	LYS276
C	LYS254
C	LYS276
D	LYS254
D	LYS276

site_id	SWS_FT_FI8
Number of Residues	4
Details	MOD_RES: Phosphothreonine => ECO:0000250\|UniProtKB:P45952

Chain	Residue	Details
A	THR326
B	THR326
C	THR326
D	THR326

Catalytic Information from CSA

site_id	CSA1
Number of Residues	1
Details	Annotated By Reference To The Literature 1ivh

Chain	Residue	Details
A	GLU255

site_id	CSA2
Number of Residues	1
Details	Annotated By Reference To The Literature 1ivh

Chain	Residue	Details
B	GLU255

site_id	CSA3
Number of Residues	1
Details	Annotated By Reference To The Literature 1ivh

Chain	Residue	Details
C	GLU255

site_id	CSA4
Number of Residues	1
Details	Annotated By Reference To The Literature 1ivh

Chain	Residue	Details
D	GLU255

site_id	CSA5
Number of Residues	1
Details	Annotated By Reference To The Literature 1ivh

Chain	Residue	Details
A	GLY376

site_id	CSA6
Number of Residues	1
Details	Annotated By Reference To The Literature 1ivh

Chain	Residue	Details
B	GLY376

site_id	CSA7
Number of Residues	1
Details	Annotated By Reference To The Literature 1ivh

Chain	Residue	Details
C	GLY376

site_id	CSA8
Number of Residues	1
Details	Annotated By Reference To The Literature 1ivh

Chain	Residue	Details
D	GLY376

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)