1ECP
PURINE NUCLEOSIDE PHOSPHORYLASE
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0004731 | molecular_function | purine-nucleoside phosphorylase activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0006139 | biological_process | nucleobase-containing compound metabolic process |
| A | 0006152 | biological_process | purine nucleoside catabolic process |
| A | 0006974 | biological_process | DNA damage response |
| A | 0009116 | biological_process | nucleoside metabolic process |
| A | 0009164 | biological_process | nucleoside catabolic process |
| A | 0016020 | cellular_component | membrane |
| A | 0016757 | molecular_function | glycosyltransferase activity |
| A | 0016763 | molecular_function | pentosyltransferase activity |
| A | 0019686 | biological_process | purine nucleoside interconversion |
| A | 0042278 | biological_process | purine nucleoside metabolic process |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0047975 | molecular_function | guanosine phosphorylase activity |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0004731 | molecular_function | purine-nucleoside phosphorylase activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0006139 | biological_process | nucleobase-containing compound metabolic process |
| B | 0006152 | biological_process | purine nucleoside catabolic process |
| B | 0006974 | biological_process | DNA damage response |
| B | 0009116 | biological_process | nucleoside metabolic process |
| B | 0009164 | biological_process | nucleoside catabolic process |
| B | 0016020 | cellular_component | membrane |
| B | 0016757 | molecular_function | glycosyltransferase activity |
| B | 0016763 | molecular_function | pentosyltransferase activity |
| B | 0019686 | biological_process | purine nucleoside interconversion |
| B | 0042278 | biological_process | purine nucleoside metabolic process |
| B | 0042802 | molecular_function | identical protein binding |
| B | 0047975 | molecular_function | guanosine phosphorylase activity |
| C | 0003824 | molecular_function | catalytic activity |
| C | 0004731 | molecular_function | purine-nucleoside phosphorylase activity |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0006139 | biological_process | nucleobase-containing compound metabolic process |
| C | 0006152 | biological_process | purine nucleoside catabolic process |
| C | 0006974 | biological_process | DNA damage response |
| C | 0009116 | biological_process | nucleoside metabolic process |
| C | 0009164 | biological_process | nucleoside catabolic process |
| C | 0016020 | cellular_component | membrane |
| C | 0016757 | molecular_function | glycosyltransferase activity |
| C | 0016763 | molecular_function | pentosyltransferase activity |
| C | 0019686 | biological_process | purine nucleoside interconversion |
| C | 0042278 | biological_process | purine nucleoside metabolic process |
| C | 0042802 | molecular_function | identical protein binding |
| C | 0047975 | molecular_function | guanosine phosphorylase activity |
| D | 0003824 | molecular_function | catalytic activity |
| D | 0004731 | molecular_function | purine-nucleoside phosphorylase activity |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005829 | cellular_component | cytosol |
| D | 0006139 | biological_process | nucleobase-containing compound metabolic process |
| D | 0006152 | biological_process | purine nucleoside catabolic process |
| D | 0006974 | biological_process | DNA damage response |
| D | 0009116 | biological_process | nucleoside metabolic process |
| D | 0009164 | biological_process | nucleoside catabolic process |
| D | 0016020 | cellular_component | membrane |
| D | 0016757 | molecular_function | glycosyltransferase activity |
| D | 0016763 | molecular_function | pentosyltransferase activity |
| D | 0019686 | biological_process | purine nucleoside interconversion |
| D | 0042278 | biological_process | purine nucleoside metabolic process |
| D | 0042802 | molecular_function | identical protein binding |
| D | 0047975 | molecular_function | guanosine phosphorylase activity |
| E | 0003824 | molecular_function | catalytic activity |
| E | 0004731 | molecular_function | purine-nucleoside phosphorylase activity |
| E | 0005737 | cellular_component | cytoplasm |
| E | 0005829 | cellular_component | cytosol |
| E | 0006139 | biological_process | nucleobase-containing compound metabolic process |
| E | 0006152 | biological_process | purine nucleoside catabolic process |
| E | 0006974 | biological_process | DNA damage response |
| E | 0009116 | biological_process | nucleoside metabolic process |
| E | 0009164 | biological_process | nucleoside catabolic process |
| E | 0016020 | cellular_component | membrane |
| E | 0016757 | molecular_function | glycosyltransferase activity |
| E | 0016763 | molecular_function | pentosyltransferase activity |
| E | 0019686 | biological_process | purine nucleoside interconversion |
| E | 0042278 | biological_process | purine nucleoside metabolic process |
| E | 0042802 | molecular_function | identical protein binding |
| E | 0047975 | molecular_function | guanosine phosphorylase activity |
| F | 0003824 | molecular_function | catalytic activity |
| F | 0004731 | molecular_function | purine-nucleoside phosphorylase activity |
| F | 0005737 | cellular_component | cytoplasm |
| F | 0005829 | cellular_component | cytosol |
| F | 0006139 | biological_process | nucleobase-containing compound metabolic process |
| F | 0006152 | biological_process | purine nucleoside catabolic process |
| F | 0006974 | biological_process | DNA damage response |
| F | 0009116 | biological_process | nucleoside metabolic process |
| F | 0009164 | biological_process | nucleoside catabolic process |
| F | 0016020 | cellular_component | membrane |
| F | 0016757 | molecular_function | glycosyltransferase activity |
| F | 0016763 | molecular_function | pentosyltransferase activity |
| F | 0019686 | biological_process | purine nucleoside interconversion |
| F | 0042278 | biological_process | purine nucleoside metabolic process |
| F | 0042802 | molecular_function | identical protein binding |
| F | 0047975 | molecular_function | guanosine phosphorylase activity |
Functional Information from PDB Data
| site_id | S1 |
| Number of Residues | 5 |
| Details | PHOSPHATE BINDING SITE. |
| Chain | Residue |
| A | GLY20 |
| A | ARG24 |
| A | ARG43 |
| A | ARG87 |
| A | SER90 |
| site_id | S2 |
| Number of Residues | 5 |
| Details | BASE BINDING SITE. |
| Chain | Residue |
| A | ILE206 |
| A | PHE159 |
| A | VAL178 |
| A | MET180 |
| A | ASP204 |
Functional Information from PROSITE/UniProt
| site_id | PS01232 |
| Number of Residues | 16 |
| Details | PNP_UDP_1 Purine and other phosphorylases family 1 signature. GhGMGiPScSIytkEL |
| Chain | Residue | Details |
| A | GLY61-LEU76 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 6 |
| Details | ACT_SITE: Proton donor => ECO:0000255|HAMAP-Rule:MF_01627, ECO:0000269|PubMed:30337572 |
| Chain | Residue | Details |
| A | HIS205 | |
| B | HIS205 | |
| C | HIS205 | |
| D | HIS205 | |
| E | HIS205 | |
| F | HIS205 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000305|PubMed:30337572, ECO:0007744|PDB:4TS3, ECO:0007744|PDB:4TS9, ECO:0007744|PDB:4TTA, ECO:0007744|PDB:4TTI, ECO:0007744|PDB:4TTJ |
| Chain | Residue | Details |
| A | ILE5 | |
| B | ILE5 | |
| C | ILE5 | |
| D | ILE5 | |
| E | ILE5 | |
| F | ILE5 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 18 |
| Details | BINDING: in other chain => ECO:0000269|PubMed:11786017, ECO:0000269|PubMed:21672603, ECO:0000269|PubMed:30337572, ECO:0007744|PDB:1K9S, ECO:0007744|PDB:3ONV, ECO:0007744|PDB:3OOE, ECO:0007744|PDB:3OOH, ECO:0007744|PDB:3OPV, ECO:0007744|PDB:4TS3, ECO:0007744|PDB:4TS9, ECO:0007744|PDB:4TTA, ECO:0007744|PDB:4TTI, ECO:0007744|PDB:4TTJ |
| Chain | Residue | Details |
| A | ASP21 | |
| D | ASP21 | |
| D | ALA25 | |
| D | VAL88 | |
| E | ASP21 | |
| E | ALA25 | |
| E | VAL88 | |
| F | ASP21 | |
| F | ALA25 | |
| F | VAL88 | |
| A | ALA25 | |
| A | VAL88 | |
| B | ASP21 | |
| B | ALA25 | |
| B | VAL88 | |
| C | ASP21 | |
| C | ALA25 | |
| C | VAL88 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:11786017, ECO:0000269|PubMed:21672603, ECO:0000269|PubMed:30337572, ECO:0007744|PDB:1K9S, ECO:0007744|PDB:3ONV, ECO:0007744|PDB:3OOE, ECO:0007744|PDB:3OOH, ECO:0007744|PDB:3OPV, ECO:0007744|PDB:4TS3, ECO:0007744|PDB:4TS9, ECO:0007744|PDB:4TTA, ECO:0007744|PDB:4TTI, ECO:0007744|PDB:4TTJ |
| Chain | Residue | Details |
| A | GLY44 | |
| B | GLY44 | |
| C | GLY44 | |
| D | GLY44 | |
| E | GLY44 | |
| F | GLY44 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 12 |
| Details | BINDING: in other chain => ECO:0000305|PubMed:30337572, ECO:0007744|PDB:4TS3, ECO:0007744|PDB:4TS9, ECO:0007744|PDB:4TTA, ECO:0007744|PDB:4TTI, ECO:0007744|PDB:4TTJ |
| Chain | Residue | Details |
| A | MET180 | |
| E | ASP204 | |
| F | MET180 | |
| F | ASP204 | |
| A | ASP204 | |
| B | MET180 | |
| B | ASP204 | |
| C | MET180 | |
| C | ASP204 | |
| D | MET180 | |
| D | ASP204 | |
| E | MET180 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 6 |
| Details | SITE: Important for catalytic activity => ECO:0000255|HAMAP-Rule:MF_01627, ECO:0000269|PubMed:21672603, ECO:0000269|PubMed:30337572 |
| Chain | Residue | Details |
| A | GLN218 | |
| B | GLN218 | |
| C | GLN218 | |
| D | GLN218 | |
| E | GLN218 | |
| F | GLN218 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 6 |
| Details | MOD_RES: N6-acetyllysine => ECO:0000269|PubMed:18723842 |
| Chain | Residue | Details |
| A | TYR27 | |
| B | TYR27 | |
| C | TYR27 | |
| D | TYR27 | |
| E | TYR27 | |
| F | TYR27 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1a69 |
| Chain | Residue | Details |
| A | ASP204 | |
| A | ARG217 |
| site_id | CSA2 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1a69 |
| Chain | Residue | Details |
| B | ASP204 | |
| B | ARG217 |
| site_id | CSA3 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1a69 |
| Chain | Residue | Details |
| C | ASP204 | |
| C | ARG217 |
| site_id | CSA4 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1a69 |
| Chain | Residue | Details |
| D | ASP204 | |
| D | ARG217 |
| site_id | CSA5 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1a69 |
| Chain | Residue | Details |
| E | ASP204 | |
| E | ARG217 |
| site_id | CSA6 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1a69 |
| Chain | Residue | Details |
| F | ASP204 | |
| F | ARG217 |
| site_id | MCSA1 |
| Number of Residues | 7 |
| Details | M-CSA 375 |
| Chain | Residue | Details |
| A | ASP21 | electrostatic stabiliser |
| A | ALA25 | electrostatic stabiliser |
| A | GLY44 | electrostatic stabiliser |
| A | VAL88 | electrostatic stabiliser |
| A | CYS91 | electrostatic stabiliser |
| A | HIS205 | proton shuttle (general acid/base) |
| A | GLN218 | enhance reactivity |
| site_id | MCSA2 |
| Number of Residues | 7 |
| Details | M-CSA 375 |
| Chain | Residue | Details |
| B | ASP21 | electrostatic stabiliser |
| B | ALA25 | electrostatic stabiliser |
| B | GLY44 | electrostatic stabiliser |
| B | VAL88 | electrostatic stabiliser |
| B | CYS91 | electrostatic stabiliser |
| B | HIS205 | proton shuttle (general acid/base) |
| B | GLN218 | enhance reactivity |
| site_id | MCSA3 |
| Number of Residues | 7 |
| Details | M-CSA 375 |
| Chain | Residue | Details |
| C | ASP21 | electrostatic stabiliser |
| C | ALA25 | electrostatic stabiliser |
| C | GLY44 | electrostatic stabiliser |
| C | VAL88 | electrostatic stabiliser |
| C | CYS91 | electrostatic stabiliser |
| C | HIS205 | proton shuttle (general acid/base) |
| C | GLN218 | enhance reactivity |
| site_id | MCSA4 |
| Number of Residues | 7 |
| Details | M-CSA 375 |
| Chain | Residue | Details |
| D | ASP21 | electrostatic stabiliser |
| D | ALA25 | electrostatic stabiliser |
| D | GLY44 | electrostatic stabiliser |
| D | VAL88 | electrostatic stabiliser |
| D | CYS91 | electrostatic stabiliser |
| D | HIS205 | proton shuttle (general acid/base) |
| D | GLN218 | enhance reactivity |
| site_id | MCSA5 |
| Number of Residues | 7 |
| Details | M-CSA 375 |
| Chain | Residue | Details |
| E | ASP21 | electrostatic stabiliser |
| E | ALA25 | electrostatic stabiliser |
| E | GLY44 | electrostatic stabiliser |
| E | VAL88 | electrostatic stabiliser |
| E | CYS91 | electrostatic stabiliser |
| E | HIS205 | proton shuttle (general acid/base) |
| E | GLN218 | enhance reactivity |
| site_id | MCSA6 |
| Number of Residues | 7 |
| Details | M-CSA 375 |
| Chain | Residue | Details |
| F | ASP21 | electrostatic stabiliser |
| F | ALA25 | electrostatic stabiliser |
| F | GLY44 | electrostatic stabiliser |
| F | VAL88 | electrostatic stabiliser |
| F | CYS91 | electrostatic stabiliser |
| F | HIS205 | proton shuttle (general acid/base) |
| F | GLN218 | enhance reactivity |
