1ECP

PURINE NUCLEOSIDE PHOSPHORYLASE

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0004731	molecular_function	purine-nucleoside phosphorylase activity
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006139	biological_process	nucleobase-containing compound metabolic process
A	0006152	biological_process	purine nucleoside catabolic process
A	0006974	biological_process	DNA damage response
A	0009116	biological_process	nucleoside metabolic process
A	0009164	biological_process	nucleoside catabolic process
A	0016020	cellular_component	membrane
A	0016740	molecular_function	transferase activity
A	0016757	molecular_function	glycosyltransferase activity
A	0016763	molecular_function	pentosyltransferase activity
A	0019686	biological_process	purine nucleoside interconversion
A	0042278	biological_process	purine nucleoside metabolic process
A	0042802	molecular_function	identical protein binding
A	0047975	molecular_function	guanosine phosphorylase activity
B	0003824	molecular_function	catalytic activity
B	0004731	molecular_function	purine-nucleoside phosphorylase activity
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006139	biological_process	nucleobase-containing compound metabolic process
B	0006152	biological_process	purine nucleoside catabolic process
B	0006974	biological_process	DNA damage response
B	0009116	biological_process	nucleoside metabolic process
B	0009164	biological_process	nucleoside catabolic process
B	0016020	cellular_component	membrane
B	0016740	molecular_function	transferase activity
B	0016757	molecular_function	glycosyltransferase activity
B	0016763	molecular_function	pentosyltransferase activity
B	0019686	biological_process	purine nucleoside interconversion
B	0042278	biological_process	purine nucleoside metabolic process
B	0042802	molecular_function	identical protein binding
B	0047975	molecular_function	guanosine phosphorylase activity
C	0003824	molecular_function	catalytic activity
C	0004731	molecular_function	purine-nucleoside phosphorylase activity
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006139	biological_process	nucleobase-containing compound metabolic process
C	0006152	biological_process	purine nucleoside catabolic process
C	0006974	biological_process	DNA damage response
C	0009116	biological_process	nucleoside metabolic process
C	0009164	biological_process	nucleoside catabolic process
C	0016020	cellular_component	membrane
C	0016740	molecular_function	transferase activity
C	0016757	molecular_function	glycosyltransferase activity
C	0016763	molecular_function	pentosyltransferase activity
C	0019686	biological_process	purine nucleoside interconversion
C	0042278	biological_process	purine nucleoside metabolic process
C	0042802	molecular_function	identical protein binding
C	0047975	molecular_function	guanosine phosphorylase activity
D	0003824	molecular_function	catalytic activity
D	0004731	molecular_function	purine-nucleoside phosphorylase activity
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006139	biological_process	nucleobase-containing compound metabolic process
D	0006152	biological_process	purine nucleoside catabolic process
D	0006974	biological_process	DNA damage response
D	0009116	biological_process	nucleoside metabolic process
D	0009164	biological_process	nucleoside catabolic process
D	0016020	cellular_component	membrane
D	0016740	molecular_function	transferase activity
D	0016757	molecular_function	glycosyltransferase activity
D	0016763	molecular_function	pentosyltransferase activity
D	0019686	biological_process	purine nucleoside interconversion
D	0042278	biological_process	purine nucleoside metabolic process
D	0042802	molecular_function	identical protein binding
D	0047975	molecular_function	guanosine phosphorylase activity
E	0003824	molecular_function	catalytic activity
E	0004731	molecular_function	purine-nucleoside phosphorylase activity
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006139	biological_process	nucleobase-containing compound metabolic process
E	0006152	biological_process	purine nucleoside catabolic process
E	0006974	biological_process	DNA damage response
E	0009116	biological_process	nucleoside metabolic process
E	0009164	biological_process	nucleoside catabolic process
E	0016020	cellular_component	membrane
E	0016740	molecular_function	transferase activity
E	0016757	molecular_function	glycosyltransferase activity
E	0016763	molecular_function	pentosyltransferase activity
E	0019686	biological_process	purine nucleoside interconversion
E	0042278	biological_process	purine nucleoside metabolic process
E	0042802	molecular_function	identical protein binding
E	0047975	molecular_function	guanosine phosphorylase activity
F	0003824	molecular_function	catalytic activity
F	0004731	molecular_function	purine-nucleoside phosphorylase activity
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006139	biological_process	nucleobase-containing compound metabolic process
F	0006152	biological_process	purine nucleoside catabolic process
F	0006974	biological_process	DNA damage response
F	0009116	biological_process	nucleoside metabolic process
F	0009164	biological_process	nucleoside catabolic process
F	0016020	cellular_component	membrane
F	0016740	molecular_function	transferase activity
F	0016757	molecular_function	glycosyltransferase activity
F	0016763	molecular_function	pentosyltransferase activity
F	0019686	biological_process	purine nucleoside interconversion
F	0042278	biological_process	purine nucleoside metabolic process
F	0042802	molecular_function	identical protein binding
F	0047975	molecular_function	guanosine phosphorylase activity

Functional Information from PDB Data

site_id	S1
Number of Residues	5
Details	PHOSPHATE BINDING SITE.

Chain	Residue
A	GLY20
A	ARG24
A	ARG43
A	ARG87
A	SER90

---

site_id	S2
Number of Residues	5
Details	BASE BINDING SITE.

Chain	Residue
A	ILE206
A	PHE159
A	VAL178
A	MET180
A	ASP204

---

Functional Information from PROSITE/UniProt

site_id	PS01232
Number of Residues	16
Details	PNP_UDP_1 Purine and other phosphorylases family 1 signature. GhGMGiPScSIytkEL

Chain	Residue	Details
A	GLY61-LEU76

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	ACT_SITE: Proton donor => ECO:0000255|HAMAP-Rule:MF_01627, ECO:0000269|PubMed:30337572

Chain	Residue	Details
A	HIS205
B	HIS205
C	HIS205
D	HIS205
E	HIS205
F	HIS205

---

site_id	SWS_FT_FI2
Number of Residues	6
Details	BINDING: BINDING => ECO:0000305|PubMed:30337572, ECO:0007744|PDB:4TS3, ECO:0007744|PDB:4TS9, ECO:0007744|PDB:4TTA, ECO:0007744|PDB:4TTI, ECO:0007744|PDB:4TTJ

Chain	Residue	Details
A	ILE5
B	ILE5
C	ILE5
D	ILE5
E	ILE5
F	ILE5

---

site_id	SWS_FT_FI3
Number of Residues	18
Details	BINDING: in other chain => ECO:0000269|PubMed:11786017, ECO:0000269|PubMed:21672603, ECO:0000269|PubMed:30337572, ECO:0007744|PDB:1K9S, ECO:0007744|PDB:3ONV, ECO:0007744|PDB:3OOE, ECO:0007744|PDB:3OOH, ECO:0007744|PDB:3OPV, ECO:0007744|PDB:4TS3, ECO:0007744|PDB:4TS9, ECO:0007744|PDB:4TTA, ECO:0007744|PDB:4TTI, ECO:0007744|PDB:4TTJ

Chain	Residue	Details
A	ASP21
D	ASP21
D	ALA25
D	VAL88
E	ASP21
E	ALA25
E	VAL88
F	ASP21
F	ALA25
F	VAL88
A	ALA25
A	VAL88
B	ASP21
B	ALA25
B	VAL88
C	ASP21
C	ALA25
C	VAL88

---

site_id	SWS_FT_FI4
Number of Residues	6
Details	BINDING: BINDING => ECO:0000269|PubMed:11786017, ECO:0000269|PubMed:21672603, ECO:0000269|PubMed:30337572, ECO:0007744|PDB:1K9S, ECO:0007744|PDB:3ONV, ECO:0007744|PDB:3OOE, ECO:0007744|PDB:3OOH, ECO:0007744|PDB:3OPV, ECO:0007744|PDB:4TS3, ECO:0007744|PDB:4TS9, ECO:0007744|PDB:4TTA, ECO:0007744|PDB:4TTI, ECO:0007744|PDB:4TTJ

Chain	Residue	Details
A	GLY44
B	GLY44
C	GLY44
D	GLY44
E	GLY44
F	GLY44

---

site_id	SWS_FT_FI5
Number of Residues	12
Details	BINDING: in other chain => ECO:0000305|PubMed:30337572, ECO:0007744|PDB:4TS3, ECO:0007744|PDB:4TS9, ECO:0007744|PDB:4TTA, ECO:0007744|PDB:4TTI, ECO:0007744|PDB:4TTJ

Chain	Residue	Details
A	MET180
E	ASP204
F	MET180
F	ASP204
A	ASP204
B	MET180
B	ASP204
C	MET180
C	ASP204
D	MET180
D	ASP204
E	MET180

---

site_id	SWS_FT_FI6
Number of Residues	6
Details	SITE: Important for catalytic activity => ECO:0000255|HAMAP-Rule:MF_01627, ECO:0000269|PubMed:21672603, ECO:0000269|PubMed:30337572

Chain	Residue	Details
A	GLN218
B	GLN218
C	GLN218
D	GLN218
E	GLN218
F	GLN218

---

site_id	SWS_FT_FI7
Number of Residues	6
Details	MOD_RES: N6-acetyllysine => ECO:0000269|PubMed:18723842

Chain	Residue	Details
A	TYR27
B	TYR27
C	TYR27
D	TYR27
E	TYR27
F	TYR27

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1a69

Chain	Residue	Details
A	ASP204
A	ARG217

---

site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1a69

Chain	Residue	Details
B	ASP204
B	ARG217

---

site_id	CSA3
Number of Residues	2
Details	Annotated By Reference To The Literature 1a69

Chain	Residue	Details
C	ASP204
C	ARG217

---

site_id	CSA4
Number of Residues	2
Details	Annotated By Reference To The Literature 1a69

Chain	Residue	Details
D	ASP204
D	ARG217

---

site_id	CSA5
Number of Residues	2
Details	Annotated By Reference To The Literature 1a69

Chain	Residue	Details
E	ASP204
E	ARG217

---

site_id	CSA6
Number of Residues	2
Details	Annotated By Reference To The Literature 1a69

Chain	Residue	Details
F	ASP204
F	ARG217

---

site_id	MCSA1
Number of Residues	7
Details	M-CSA 375

Chain	Residue	Details
A	ASP21	electrostatic stabiliser
A	ALA25	electrostatic stabiliser
A	GLY44	electrostatic stabiliser
A	VAL88	electrostatic stabiliser
A	CYS91	electrostatic stabiliser
A	HIS205	proton shuttle (general acid/base)
A	GLN218	enhance reactivity

---

site_id	MCSA2
Number of Residues	7
Details	M-CSA 375

Chain	Residue	Details
B	ASP21	electrostatic stabiliser
B	ALA25	electrostatic stabiliser
B	GLY44	electrostatic stabiliser
B	VAL88	electrostatic stabiliser
B	CYS91	electrostatic stabiliser
B	HIS205	proton shuttle (general acid/base)
B	GLN218	enhance reactivity

---

site_id	MCSA3
Number of Residues	7
Details	M-CSA 375

Chain	Residue	Details
C	ASP21	electrostatic stabiliser
C	ALA25	electrostatic stabiliser
C	GLY44	electrostatic stabiliser
C	VAL88	electrostatic stabiliser
C	CYS91	electrostatic stabiliser
C	HIS205	proton shuttle (general acid/base)
C	GLN218	enhance reactivity

---

site_id	MCSA4
Number of Residues	7
Details	M-CSA 375

Chain	Residue	Details
D	ASP21	electrostatic stabiliser
D	ALA25	electrostatic stabiliser
D	GLY44	electrostatic stabiliser
D	VAL88	electrostatic stabiliser
D	CYS91	electrostatic stabiliser
D	HIS205	proton shuttle (general acid/base)
D	GLN218	enhance reactivity

---

site_id	MCSA5
Number of Residues	7
Details	M-CSA 375

Chain	Residue	Details
E	ASP21	electrostatic stabiliser
E	ALA25	electrostatic stabiliser
E	GLY44	electrostatic stabiliser
E	VAL88	electrostatic stabiliser
E	CYS91	electrostatic stabiliser
E	HIS205	proton shuttle (general acid/base)
E	GLN218	enhance reactivity

---

site_id	MCSA6
Number of Residues	7
Details	M-CSA 375

Chain	Residue	Details
F	ASP21	electrostatic stabiliser
F	ALA25	electrostatic stabiliser
F	GLY44	electrostatic stabiliser
F	VAL88	electrostatic stabiliser
F	CYS91	electrostatic stabiliser
F	HIS205	proton shuttle (general acid/base)
F	GLN218	enhance reactivity

---