1ECP

PURINE NUCLEOSIDE PHOSPHORYLASE

Experimental procedure

Detector technology	AREA DETECTOR
Detector	XUONG-HAMLIN MULTIWIRE MARK II
Spacegroup name	P 1 21 1
Unit cell lengths	89.500, 110.600, 74.600
Unit cell angles	90.00, 111.70, 90.00

Refinement procedure

Resolution	8.000 * - 2.000
R-factor	0.2
Rwork	0.200
R-free	0.29000
RMSD bond length	0.005 *
RMSD bond angle	1.300 *
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
High resolution limit [Å]	2.000 *
Rmerge	0.087
Total number of observations	230200 *
Number of reflections	70600 *
Completeness [%]	70.0
Redundancy	3.26

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	12-14 (%)
2	1	reservoir	citrate	50 (mM)