1EBU
HOMOSERINE DEHYDROGENASE COMPLEX WITH NAD ANALOGUE AND L-HOMOSERINE
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004412 | molecular_function | homoserine dehydrogenase activity |
| A | 0005634 | cellular_component | nucleus |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0006520 | biological_process | amino acid metabolic process |
| A | 0008652 | biological_process | amino acid biosynthetic process |
| A | 0009067 | biological_process | aspartate family amino acid biosynthetic process |
| A | 0009086 | biological_process | methionine biosynthetic process |
| A | 0009088 | biological_process | threonine biosynthetic process |
| A | 0009090 | biological_process | homoserine biosynthetic process |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0050661 | molecular_function | NADP binding |
| A | 0070403 | molecular_function | NAD+ binding |
| B | 0004412 | molecular_function | homoserine dehydrogenase activity |
| B | 0005634 | cellular_component | nucleus |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0006520 | biological_process | amino acid metabolic process |
| B | 0008652 | biological_process | amino acid biosynthetic process |
| B | 0009067 | biological_process | aspartate family amino acid biosynthetic process |
| B | 0009086 | biological_process | methionine biosynthetic process |
| B | 0009088 | biological_process | threonine biosynthetic process |
| B | 0009090 | biological_process | homoserine biosynthetic process |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0050661 | molecular_function | NADP binding |
| B | 0070403 | molecular_function | NAD+ binding |
| C | 0004412 | molecular_function | homoserine dehydrogenase activity |
| C | 0005634 | cellular_component | nucleus |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0006520 | biological_process | amino acid metabolic process |
| C | 0008652 | biological_process | amino acid biosynthetic process |
| C | 0009067 | biological_process | aspartate family amino acid biosynthetic process |
| C | 0009086 | biological_process | methionine biosynthetic process |
| C | 0009088 | biological_process | threonine biosynthetic process |
| C | 0009090 | biological_process | homoserine biosynthetic process |
| C | 0016491 | molecular_function | oxidoreductase activity |
| C | 0050661 | molecular_function | NADP binding |
| C | 0070403 | molecular_function | NAD+ binding |
| D | 0004412 | molecular_function | homoserine dehydrogenase activity |
| D | 0005634 | cellular_component | nucleus |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0006520 | biological_process | amino acid metabolic process |
| D | 0008652 | biological_process | amino acid biosynthetic process |
| D | 0009067 | biological_process | aspartate family amino acid biosynthetic process |
| D | 0009086 | biological_process | methionine biosynthetic process |
| D | 0009088 | biological_process | threonine biosynthetic process |
| D | 0009090 | biological_process | homoserine biosynthetic process |
| D | 0016491 | molecular_function | oxidoreductase activity |
| D | 0050661 | molecular_function | NADP binding |
| D | 0070403 | molecular_function | NAD+ binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE NA A 901 |
| Chain | Residue |
| A | GLU143 |
| A | GLY147 |
| A | ALA148 |
| A | LEU150 |
| site_id | AC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE NA B 902 |
| Chain | Residue |
| B | GLU143 |
| B | VAL146 |
| B | ALA148 |
| B | LEU150 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE NA C 903 |
| Chain | Residue |
| C | VAL146 |
| C | GLY147 |
| C | ALA148 |
| C | GLY149 |
| C | LEU150 |
| C | GLU143 |
| site_id | AC4 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE NA D 904 |
| Chain | Residue |
| D | GLU143 |
| D | VAL146 |
| D | ALA148 |
| D | LEU150 |
| site_id | AC5 |
| Number of Residues | 18 |
| Details | BINDING SITE FOR RESIDUE NDA D 1300 |
| Chain | Residue |
| D | GLY12 |
| D | ALA13 |
| D | GLY14 |
| D | VAL15 |
| D | VAL16 |
| D | GLY17 |
| D | GLU40 |
| D | ALA41 |
| D | ASN92 |
| D | THR93 |
| D | SER94 |
| D | PRO115 |
| D | LYS117 |
| D | GLY338 |
| D | ALA339 |
| D | GLY340 |
| D | THR344 |
| D | HSE1301 |
| site_id | AC6 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE HSE D 1301 |
| Chain | Residue |
| D | GLU208 |
| D | ASP219 |
| D | NDA1300 |
Functional Information from PROSITE/UniProt
| site_id | PS01042 |
| Number of Residues | 23 |
| Details | HOMOSER_DHGENASE Homoserine dehydrogenase signature. AkklGYTep.DPrdDLnGlDvarK |
| Chain | Residue | Details |
| A | ALA201-LYS223 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: Proton donor => ECO:0000255|PIRSR:PIRSR036497-1 |
| Chain | Residue | Details |
| A | LYS223 | |
| B | LYS223 | |
| C | LYS223 | |
| D | LYS223 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 12 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:10700284, ECO:0007744|PDB:1EBF, ECO:0007744|PDB:1EBU |
| Chain | Residue | Details |
| A | ALA13 | |
| D | ALA13 | |
| D | VAL15 | |
| D | ALA148 | |
| A | VAL15 | |
| A | ALA148 | |
| B | ALA13 | |
| B | VAL15 | |
| B | ALA148 | |
| C | ALA13 | |
| C | VAL15 | |
| C | ALA148 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 24 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:O58802 |
| Chain | Residue | Details |
| A | VAL16 | |
| B | SER94 | |
| B | LYS117 | |
| B | GLY340 | |
| C | VAL16 | |
| C | LYS60 | |
| C | THR93 | |
| C | SER94 | |
| C | LYS117 | |
| C | GLY340 | |
| D | VAL16 | |
| A | LYS60 | |
| D | LYS60 | |
| D | THR93 | |
| D | SER94 | |
| D | LYS117 | |
| D | GLY340 | |
| A | THR93 | |
| A | SER94 | |
| A | LYS117 | |
| A | GLY340 | |
| B | VAL16 | |
| B | LYS60 | |
| B | THR93 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:10700284, ECO:0007744|PDB:1EBF |
| Chain | Residue | Details |
| A | ALA41 | |
| B | ALA41 | |
| C | ALA41 | |
| D | ALA41 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 12 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:10700284, ECO:0000269|PubMed:14583265, ECO:0007744|PDB:1EBF, ECO:0007744|PDB:1EBU, ECO:0007744|PDB:1Q7G |
| Chain | Residue | Details |
| A | GLU143 | |
| D | GLU143 | |
| D | VAL146 | |
| D | LEU150 | |
| A | VAL146 | |
| A | LEU150 | |
| B | GLU143 | |
| B | VAL146 | |
| B | LEU150 | |
| C | GLU143 | |
| C | VAL146 | |
| C | LEU150 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 8 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:F9VNG5 |
| Chain | Residue | Details |
| A | GLY205 | |
| A | GLU208 | |
| B | GLY205 | |
| B | GLU208 | |
| C | GLY205 | |
| C | GLU208 | |
| D | GLY205 | |
| D | GLU208 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:10700284, ECO:0007744|PDB:1EBU |
| Chain | Residue | Details |
| A | ASP219 | |
| B | ASP219 | |
| C | ASP219 | |
| D | ASP219 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 8 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0007744|PubMed:22106047 |
| Chain | Residue | Details |
| A | LYS290 | |
| B | LYS290 | |
| C | LYS290 | |
| D | LYS290 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1ebf |
| Chain | Residue | Details |
| A | LYS223 | |
| A | ASP219 |
| site_id | CSA2 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1ebf |
| Chain | Residue | Details |
| B | LYS223 | |
| B | ASP219 |
| site_id | CSA3 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1ebf |
| Chain | Residue | Details |
| C | LYS223 | |
| C | ASP219 |
| site_id | CSA4 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1ebf |
| Chain | Residue | Details |
| D | LYS223 | |
| D | ASP219 |
| site_id | MCSA1 |
| Number of Residues | 2 |
| Details | M-CSA 521 |
| Chain | Residue | Details |
| A | ASP219 | electrostatic stabiliser |
| A | LYS223 | proton shuttle (general acid/base) |
| site_id | MCSA2 |
| Number of Residues | 2 |
| Details | M-CSA 521 |
| Chain | Residue | Details |
| B | ASP219 | electrostatic stabiliser |
| B | LYS223 | proton shuttle (general acid/base) |
| site_id | MCSA3 |
| Number of Residues | 2 |
| Details | M-CSA 521 |
| Chain | Residue | Details |
| C | ASP219 | electrostatic stabiliser |
| C | LYS223 | proton shuttle (general acid/base) |
| site_id | MCSA4 |
| Number of Residues | 2 |
| Details | M-CSA 521 |
| Chain | Residue | Details |
| D | ASP219 | electrostatic stabiliser |
| D | LYS223 | proton shuttle (general acid/base) |
