1EBU

HOMOSERINE DEHYDROGENASE COMPLEX WITH NAD ANALOGUE AND L-HOMOSERINE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	218
Detector technology	IMAGE PLATE
Collection date	1996-02-22
Detector	RIGAKU RAXIS II
Spacegroup name	P 1 21 1
Unit cell lengths	58.780, 104.090, 120.640
Unit cell angles	90.00, 91.93, 90.00

Refinement procedure

Resolution	39.400 - 2.600
R-factor	0.215
Rwork	0.215
R-free	0.28300
RMSD bond length	0.008
RMSD bond angle	23.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.081	0.344
Number of reflections	164716
<I/σ(I)>	13.3
Completeness [%]	99.2	98.1
Redundancy	3.7	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5 *	295	PEG 8K, L-homoserine, 3-aminopyridine dinucleotide, sodium cacodylate , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)	or 10 mM HEPES
3	1	drop		20 (mM)
4	1	reservoir	ammonium sulfate	2.0 (M)
5	1	reservoir	Tris-HCl	0.1 (M)