1E7R

GDP 4-keto-6-deoxy-D-mannose epimerase reductase Y136E

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005737	cellular_component	cytoplasm
A	0009226	biological_process	nucleotide-sugar biosynthetic process
A	0009242	biological_process	colanic acid biosynthetic process
A	0016491	molecular_function	oxidoreductase activity
A	0016853	molecular_function	isomerase activity
A	0042351	biological_process	'de novo' GDP-L-fucose biosynthetic process
A	0042803	molecular_function	protein homodimerization activity
A	0050577	molecular_function	GDP-L-fucose synthase activity
A	0070401	molecular_function	NADP+ binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 1320

Chain	Residue
A	HIS11
A	ARG12
A	ARG20
A	THR35

---

site_id	AC2
Number of Residues	9
Details	BINDING SITE FOR RESIDUE SO4 A 1321

Chain	Residue
A	PHE272
A	HOH2343
A	HOH2344
A	HOH2345
A	ARG152
A	PRO238
A	ARG254
A	VAL270
A	VAL271

---

site_id	AC3
Number of Residues	33
Details	BINDING SITE FOR RESIDUE NAP A 1317

Chain	Residue
A	GLY10
A	GLY13
A	MET14
A	VAL15
A	ARG36
A	LEU39
A	ASN40
A	LEU41
A	LEU42
A	ALA62
A	ALA63
A	ALA64
A	VAL66
A	ILE86
A	LEU105
A	GLY106
A	SER107
A	LYS140
A	PRO163
A	THR164
A	LEU166
A	HOH2101
A	HOH2323
A	HOH2324
A	HOH2325
A	HOH2326
A	HOH2327
A	HOH2328
A	HOH2329
A	HOH2330
A	HOH2331
A	HOH2332
A	HOH2333

---

site_id	AC4
Number of Residues	9
Details	BINDING SITE FOR RESIDUE UVW A 1318

Chain	Residue
A	GLY67
A	GLY68
A	ILE69
A	VAL70
A	ALA71
A	SER178
A	LYS262
A	HOH2335
A	HOH2336

---

site_id	AC5
Number of Residues	13
Details	BINDING SITE FOR RESIDUE TRS A 1319

Chain	Residue
A	ASP78
A	TYR81
A	GLN82
A	HIS92
A	HIS95
A	TYR150
A	GLN153
A	TYR154
A	HOH2337
A	HOH2338
A	HOH2339
A	HOH2341
A	HOH2342

---

site_id	AC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE TRS A 1322

Chain	Residue
A	ARG20
A	ARG21
A	HIS170
A	TRP311
A	HOH2346
A	HOH2347

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Proton donor/acceptor => ECO:0000255|HAMAP-Rule:MF_00956, ECO:0000269|PubMed:11021971

Chain	Residue	Details
A	GLU136

---

site_id	SWS_FT_FI2
Number of Residues	6
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00956, ECO:0000269|PubMed:11021971, ECO:0000269|PubMed:9817848, ECO:0000269|PubMed:9862812

Chain	Residue	Details
A	ARG36
A	LEU105
A	LYS140
A	PRO163
A	HIS179
A	GLY10

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	BINDING: BINDING => ECO:0000305

Chain	Residue	Details
A	ARG187

---

site_id	SWS_FT_FI4
Number of Residues	3
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00956

Chain	Residue	Details
A	ARG209
A	ASP278
A	TRP202

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	SITE: Important for catalytic activity

Chain	Residue	Details
A	SER107
A	CYS109

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	SITE: Lowers pKa of active site Tyr

Chain	Residue	Details
A	LYS140

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	6
Details	M-CSA 227

Chain	Residue	Details
A	SER107	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, increase acidity, increase basicity, proton acceptor, proton donor
A	SER108	electrostatic stabiliser, hydrogen bond donor
A	CYS109	electrostatic stabiliser, hydrogen bond donor, proton acceptor
A	GLU136	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, proton relay
A	LYS140	electrostatic stabiliser, hydrogen bond donor, increase acidity, increase basicity
A	HIS179	hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor

---