1E7R

GDP 4-keto-6-deoxy-D-mannose epimerase reductase Y136E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector	MARRESEARCH
Spacegroup name	P 32 2 1
Unit cell lengths	103.300, 103.300, 75.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.600
Rwork	0.139
R-free	0.18000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bws
RMSD bond length	0.017
RMSD bond angle	0.038
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.043
Number of reflections	61196
<I/σ(I)>	9.65	2.4
Completeness [%]	99.3	99.1
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.5	21 *	1.5 M LITHIUM SULPHATE, PH 6.5 0.1M TRIS BUFFER 21C

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	11-20 (mg/ml)
2	1	2	lithium sulfate	1.5 (M)
3	1	2	MES	0.1 (M)	or Tris