Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

1E5Q

Ternary complex of saccharopine reductase from Magnaporthe grisea, NADPH and saccharopine

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004753	molecular_function	saccharopine dehydrogenase activity
A	0004755	molecular_function	saccharopine dehydrogenase (NADP+, L-glutamate-forming) activity
A	0005575	cellular_component	cellular_component
A	0005737	cellular_component	cytoplasm
A	0006553	biological_process	lysine metabolic process
A	0008652	biological_process	amino acid biosynthetic process
A	0009085	biological_process	lysine biosynthetic process
A	0016491	molecular_function	oxidoreductase activity
A	0019878	biological_process	lysine biosynthetic process via aminoadipic acid
B	0004753	molecular_function	saccharopine dehydrogenase activity
B	0004755	molecular_function	saccharopine dehydrogenase (NADP+, L-glutamate-forming) activity
B	0005575	cellular_component	cellular_component
B	0005737	cellular_component	cytoplasm
B	0006553	biological_process	lysine metabolic process
B	0008652	biological_process	amino acid biosynthetic process
B	0009085	biological_process	lysine biosynthetic process
B	0016491	molecular_function	oxidoreductase activity
B	0019878	biological_process	lysine biosynthetic process via aminoadipic acid
C	0004753	molecular_function	saccharopine dehydrogenase activity
C	0004755	molecular_function	saccharopine dehydrogenase (NADP+, L-glutamate-forming) activity
C	0005575	cellular_component	cellular_component
C	0005737	cellular_component	cytoplasm
C	0006553	biological_process	lysine metabolic process
C	0008652	biological_process	amino acid biosynthetic process
C	0009085	biological_process	lysine biosynthetic process
C	0016491	molecular_function	oxidoreductase activity
C	0019878	biological_process	lysine biosynthetic process via aminoadipic acid
D	0004753	molecular_function	saccharopine dehydrogenase activity
D	0004755	molecular_function	saccharopine dehydrogenase (NADP+, L-glutamate-forming) activity
D	0005575	cellular_component	cellular_component
D	0005737	cellular_component	cytoplasm
D	0006553	biological_process	lysine metabolic process
D	0008652	biological_process	amino acid biosynthetic process
D	0009085	biological_process	lysine biosynthetic process
D	0016491	molecular_function	oxidoreductase activity
D	0019878	biological_process	lysine biosynthetic process via aminoadipic acid
E	0004753	molecular_function	saccharopine dehydrogenase activity
E	0004755	molecular_function	saccharopine dehydrogenase (NADP+, L-glutamate-forming) activity
E	0005575	cellular_component	cellular_component
E	0005737	cellular_component	cytoplasm
E	0006553	biological_process	lysine metabolic process
E	0008652	biological_process	amino acid biosynthetic process
E	0009085	biological_process	lysine biosynthetic process
E	0016491	molecular_function	oxidoreductase activity
E	0019878	biological_process	lysine biosynthetic process via aminoadipic acid
F	0004753	molecular_function	saccharopine dehydrogenase activity
F	0004755	molecular_function	saccharopine dehydrogenase (NADP+, L-glutamate-forming) activity
F	0005575	cellular_component	cellular_component
F	0005737	cellular_component	cytoplasm
F	0006553	biological_process	lysine metabolic process
F	0008652	biological_process	amino acid biosynthetic process
F	0009085	biological_process	lysine biosynthetic process
F	0016491	molecular_function	oxidoreductase activity
F	0019878	biological_process	lysine biosynthetic process via aminoadipic acid
G	0004753	molecular_function	saccharopine dehydrogenase activity
G	0004755	molecular_function	saccharopine dehydrogenase (NADP+, L-glutamate-forming) activity
G	0005575	cellular_component	cellular_component
G	0005737	cellular_component	cytoplasm
G	0006553	biological_process	lysine metabolic process
G	0008652	biological_process	amino acid biosynthetic process
G	0009085	biological_process	lysine biosynthetic process
G	0016491	molecular_function	oxidoreductase activity
G	0019878	biological_process	lysine biosynthetic process via aminoadipic acid
H	0004753	molecular_function	saccharopine dehydrogenase activity
H	0004755	molecular_function	saccharopine dehydrogenase (NADP+, L-glutamate-forming) activity
H	0005575	cellular_component	cellular_component
H	0005737	cellular_component	cytoplasm
H	0006553	biological_process	lysine metabolic process
H	0008652	biological_process	amino acid biosynthetic process
H	0009085	biological_process	lysine biosynthetic process
H	0016491	molecular_function	oxidoreductase activity
H	0019878	biological_process	lysine biosynthetic process via aminoadipic acid

Functional Information from PDB Data

site_id	AC1
Number of Residues	33
Details	BINDING SITE FOR RESIDUE NDP A 500

Chain	Residue
A	SER11
A	VAL56
A	LEU75
A	ILE76
A	PRO77
A	TYR78
A	PHE80
A	THR98
A	SER99
A	GLY124
A	LEU125
A	GLY12
A	ASP126
A	PRO127
A	TRP174
A	SER175
A	VAL399
A	SHR501
A	HOH2062
A	HOH2293
A	HOH2294
A	HOH2295
A	PHE13
A	HOH2296
A	HOH2297
A	HOH2298
A	HOH2299
A	VAL14
A	CYS33
A	ARG34
A	THR35
A	LEU54
A	ASP55

site_id	AC2
Number of Residues	17
Details	BINDING SITE FOR RESIDUE SHR A 501

Chain	Residue
A	TYR78
A	SER99
A	TYR100
A	ASP126
A	CYS155
A	GLY156
A	GLY157
A	TRP174
A	ARG224
A	THR245
A	ARG247
A	NDP500
A	HOH2085
A	HOH2300
A	HOH2301
A	HOH2302
A	HOH2303

site_id	AC3
Number of Residues	34
Details	BINDING SITE FOR RESIDUE NDP B 500

Chain	Residue
B	SER11
B	GLY12
B	PHE13
B	VAL14
B	CYS33
B	ARG34
B	THR35
B	LEU54
B	ASP55
B	VAL56
B	ASN57
B	LEU75
B	ILE76
B	PRO77
B	TYR78
B	PHE80
B	THR98
B	SER99
B	GLY124
B	LEU125
B	ASP126
B	PRO127
B	TRP174
B	SER175
B	VAL399
B	SHR501
B	HOH2289
B	HOH2290
B	HOH2291
B	HOH2292
B	HOH2293
B	HOH2294
B	HOH2295
B	HOH2296

site_id	AC4
Number of Residues	17
Details	BINDING SITE FOR RESIDUE SHR B 501

Chain	Residue
B	HOH2297
B	HOH2298
B	TYR78
B	SER99
B	TYR100
B	ASP126
B	CYS155
B	GLY156
B	GLY157
B	TRP174
B	ARG224
B	THR245
B	ARG247
B	NDP500
B	HOH2103
B	HOH2150
B	HOH2160

site_id	AC5
Number of Residues	34
Details	BINDING SITE FOR RESIDUE NDP C 500

Chain	Residue
C	SER11
C	GLY12
C	PHE13
C	VAL14
C	CYS33
C	ARG34
C	THR35
C	LEU54
C	ASP55
C	VAL56
C	ASN57
C	LEU75
C	ILE76
C	PRO77
C	TYR78
C	PHE80
C	THR98
C	SER99
C	LEU125
C	ASP126
C	PRO127
C	TRP174
C	SER175
C	VAL399
C	SHR501
C	HOH2339
C	HOH2340
C	HOH2341
C	HOH2342
C	HOH2343
C	HOH2344
C	HOH2345
C	HOH2346
C	HOH2347

site_id	AC6
Number of Residues	16
Details	BINDING SITE FOR RESIDUE SHR C 501

Chain	Residue
C	TYR78
C	SER99
C	TYR100
C	ASP126
C	CYS155
C	GLY156
C	GLY157
C	TRP174
C	ARG224
C	THR245
C	ARG247
C	NDP500
C	HOH2141
C	HOH2173
C	HOH2348
C	HOH2349

site_id	AC7
Number of Residues	33
Details	BINDING SITE FOR RESIDUE NDP D 500

Chain	Residue
D	SER11
D	GLY12
D	PHE13
D	VAL14
D	CYS33
D	ARG34
D	THR35
D	LEU54
D	ASP55
D	VAL56
D	ASN57
D	LEU75
D	ILE76
D	PRO77
D	TYR78
D	PHE80
D	THR98
D	SER99
D	LEU125
D	ASP126
D	PRO127
D	TRP174
D	SER175
D	VAL399
D	SHR501
D	HOH2308
D	HOH2309
D	HOH2310
D	HOH2311
D	HOH2312
D	HOH2313
D	HOH2314
D	HOH2315

site_id	AC8
Number of Residues	17
Details	BINDING SITE FOR RESIDUE SHR D 501

Chain	Residue
D	TYR78
D	SER99
D	TYR100
D	ASP126
D	CYS155
D	GLY156
D	GLY157
D	TRP174
D	ARG224
D	THR245
D	ARG247
D	NDP500
D	HOH2087
D	HOH2143
D	HOH2316
D	HOH2317
D	HOH2318

site_id	AC9
Number of Residues	35
Details	BINDING SITE FOR RESIDUE NDP E 500

Chain	Residue
E	SER11
E	GLY12
E	PHE13
E	VAL14
E	CYS33
E	ARG34
E	THR35
E	LEU54
E	ASP55
E	VAL56
E	ASN57
E	LEU75
E	ILE76
E	PRO77
E	TYR78
E	PHE80
E	THR98
E	SER99
E	GLY124
E	LEU125
E	ASP126
E	PRO127
E	TRP174
E	SER175
E	VAL399
E	SHR501
E	HOH2284
E	HOH2285
E	HOH2286
E	HOH2288
E	HOH2289
E	HOH2290
E	HOH2291
E	HOH2292
E	HOH2293

site_id	BC1
Number of Residues	17
Details	BINDING SITE FOR RESIDUE SHR E 501

Chain	Residue
E	TYR78
E	SER99
E	TYR100
E	ASP126
E	CYS155
E	GLY156
E	GLY157
E	TRP174
E	ARG224
E	THR245
E	ARG247
E	NDP500
E	HOH2112
E	HOH2151
E	HOH2294
E	HOH2295
E	HOH2296

site_id	BC2
Number of Residues	34
Details	BINDING SITE FOR RESIDUE NDP F 500

Chain	Residue
F	SER11
F	GLY12
F	PHE13
F	VAL14
F	CYS33
F	ARG34
F	THR35
F	LEU54
F	ASP55
F	VAL56
F	LEU75
F	ILE76
F	PRO77
F	TYR78
F	PHE80
F	THR98
F	SER99
F	GLY124
F	LEU125
F	ASP126
F	PRO127
F	TRP174
F	SER175
F	VAL399
F	SHR501
F	HOH2065
F	HOH2331
F	HOH2332
F	HOH2333
F	HOH2334
F	HOH2335
F	HOH2336
F	HOH2337
F	HOH2338

site_id	BC3
Number of Residues	17
Details	BINDING SITE FOR RESIDUE SHR F 501

Chain	Residue
F	TYR78
F	SER99
F	TYR100
F	ASP126
F	CYS155
F	GLY156
F	GLY157
F	TRP174
F	ARG224
F	THR245
F	ARG247
F	NDP500
F	HOH2091
F	HOH2159
F	HOH2339
F	HOH2340
F	HOH2341

site_id	BC4
Number of Residues	36
Details	BINDING SITE FOR RESIDUE NDP G 500

Chain	Residue
G	SER11
G	GLY12
G	PHE13
G	VAL14
G	CYS33
G	ARG34
G	THR35
G	LEU54
G	ASP55
G	VAL56
G	ASN57
G	LEU75
G	ILE76
G	PRO77
G	TYR78
G	PHE80
G	THR98
G	SER99
G	LEU125
G	ASP126
G	PRO127
G	TRP174
G	SER175
G	VAL399
G	SHR501
G	HOH2048
G	HOH2064
G	HOH2325
G	HOH2326
G	HOH2327
G	HOH2328
G	HOH2329
G	HOH2330
G	HOH2331
G	HOH2332
G	HOH2333

site_id	BC5
Number of Residues	17
Details	BINDING SITE FOR RESIDUE SHR G 501

Chain	Residue
G	TYR78
G	SER99
G	TYR100
G	ASP126
G	CYS155
G	GLY156
G	GLY157
G	TRP174
G	ARG224
G	THR245
G	ARG247
G	NDP500
G	HOH2091
G	HOH2151
G	HOH2158
G	HOH2334
G	HOH2335

site_id	BC6
Number of Residues	34
Details	BINDING SITE FOR RESIDUE NDP H 500

Chain	Residue
H	SER11
H	GLY12
H	PHE13
H	VAL14
H	CYS33
H	ARG34
H	THR35
H	LEU54
H	ASP55
H	VAL56
H	ASN57
H	LEU75
H	ILE76
H	PRO77
H	TYR78
H	PHE80
H	THR98
H	SER99
H	GLY124
H	LEU125
H	ASP126
H	PRO127
H	TRP174
H	SER175
H	VAL399
H	SHR501
H	HOH2259
H	HOH2260
H	HOH2261
H	HOH2262
H	HOH2264
H	HOH2265
H	HOH2266
H	HOH2267

site_id	BC7
Number of Residues	17
Details	BINDING SITE FOR RESIDUE SHR H 501

Chain	Residue
H	TYR78
H	SER99
H	TYR100
H	ASP126
H	CYS155
H	GLY156
H	GLY157
H	TRP174
H	ARG224
H	THR245
H	ARG247
H	NDP500
H	HOH2097
H	HOH2143
H	HOH2152
H	HOH2268
H	HOH2269

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	88
Details	BINDING: BINDING => ECO:0000269\|PubMed:11080625, ECO:0007744\|PDB:1E5Q

Chain	Residue	Details
A	SER11
A	ARG224
A	THR245
B	SER11
B	CYS33
B	ASP55
B	ILE76
B	THR98
B	SER99
B	LEU125
B	ASP126
A	CYS33
B	SER175
B	ARG224
B	THR245
C	SER11
C	CYS33
C	ASP55
C	ILE76
C	THR98
C	SER99
C	LEU125
A	ASP55
C	ASP126
C	SER175
C	ARG224
C	THR245
D	SER11
D	CYS33
D	ASP55
D	ILE76
D	THR98
D	SER99
A	ILE76
D	LEU125
D	ASP126
D	SER175
D	ARG224
D	THR245
E	SER11
E	CYS33
E	ASP55
E	ILE76
E	THR98
A	THR98
E	SER99
E	LEU125
E	ASP126
E	SER175
E	ARG224
E	THR245
F	SER11
F	CYS33
F	ASP55
F	ILE76
A	SER99
F	THR98
F	SER99
F	LEU125
F	ASP126
F	SER175
F	ARG224
F	THR245
G	SER11
G	CYS33
G	ASP55
A	LEU125
G	ILE76
G	THR98
G	SER99
G	LEU125
G	ASP126
G	SER175
G	ARG224
G	THR245
H	SER11
H	CYS33
A	ASP126
H	ASP55
H	ILE76
H	THR98
H	SER99
H	LEU125
H	ASP126
H	SER175
H	ARG224
H	THR245
A	SER175

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	1
Details	M-CSA 618

Chain	Residue	Details
A	ASP126

site_id	MCSA2
Number of Residues	1
Details	M-CSA 618

Chain	Residue	Details
B	ASP126

site_id	MCSA3
Number of Residues	1
Details	M-CSA 618

Chain	Residue	Details
C	ASP126

site_id	MCSA4
Number of Residues	1
Details	M-CSA 618

Chain	Residue	Details
D	ASP126

site_id	MCSA5
Number of Residues	1
Details	M-CSA 618

Chain	Residue	Details
E	ASP126

site_id	MCSA6
Number of Residues	1
Details	M-CSA 618

Chain	Residue	Details
F	ASP126

site_id	MCSA7
Number of Residues	1
Details	M-CSA 618

Chain	Residue	Details
G	ASP126

site_id	MCSA8
Number of Residues	1
Details	M-CSA 618

Chain	Residue	Details
H	ASP126

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)