1E5Q
Ternary complex of saccharopine reductase from Magnaporthe grisea, NADPH and saccharopine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-02-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.643, 205.809, 125.599 |
| Unit cell angles | 90.00, 100.05, 90.00 |
Refinement procedure
| Resolution | 19.960 - 2.100 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ff9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 23.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.065 | 0.371 |
| Total number of observations | 2072876 * | |
| Number of reflections | 250975 | |
| <I/σ(I)> | 18 | 2.5 |
| Completeness [%] | 99.7 | 96.5 |
| Redundancy | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.25 | 4 * | 8 MG/ML PROTEIN, 1 MM SACCHAROPINE, 1 MM NADPH, 20 % (W/W) PEG8000, 0.1 M TRIS-HCL PH 7.25, 1 MM DTT |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | drop | NADPH | 1 (mM) | |
| 3 | 1 | drop | saccharopine | 9 (mM) | |
| 4 | 1 | reservoir | PEG8000 | 20 (%(w/w)) | |
| 5 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
| 6 | 1 | reservoir | dithiothreitol | 1 (mM) |
