1DNP

STRUCTURE OF DEOXYRIBODIPYRIMIDINE PHOTOLYASE

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0000719	biological_process	photoreactive repair
A	0003677	molecular_function	DNA binding
A	0003684	molecular_function	damaged DNA binding
A	0003904	molecular_function	deoxyribodipyrimidine photo-lyase activity
A	0005515	molecular_function	protein binding
A	0006139	biological_process	nucleobase-containing compound metabolic process
A	0006281	biological_process	DNA repair
A	0006950	biological_process	response to stress
A	0007603	biological_process	phototransduction, visible light
A	0009416	biological_process	response to light stimulus
A	0016829	molecular_function	lyase activity
A	0071949	molecular_function	FAD binding
A	0097159	molecular_function	organic cyclic compound binding
B	0000166	molecular_function	nucleotide binding
B	0000719	biological_process	photoreactive repair
B	0003677	molecular_function	DNA binding
B	0003684	molecular_function	damaged DNA binding
B	0003904	molecular_function	deoxyribodipyrimidine photo-lyase activity
B	0005515	molecular_function	protein binding
B	0006139	biological_process	nucleobase-containing compound metabolic process
B	0006281	biological_process	DNA repair
B	0006950	biological_process	response to stress
B	0007603	biological_process	phototransduction, visible light
B	0009416	biological_process	response to light stimulus
B	0016829	molecular_function	lyase activity
B	0071949	molecular_function	FAD binding
B	0097159	molecular_function	organic cyclic compound binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	27
Details	BINDING SITE FOR RESIDUE FAD A 472

Chain	Residue
A	TYR222
A	TYR281
A	TRP338
A	ASN341
A	ARG344
A	LEU370
A	ASP372
A	GLY373
A	ASP374
A	ALA377
A	ASN378
A	THR234
A	GLY381
A	TRP382
A	HOH514
A	HOH526
A	HOH617
A	HOH704
A	HOH747
A	HOH835
A	SER235
A	ARG236
A	LEU237
A	SER238
A	TRP271
A	GLU274
A	ARG278

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site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MHF A 473

Chain	Residue
A	HIS44
A	GLU106
A	ASN108
A	GLU109
A	CYS292
A	LYS293

---

site_id	AC3
Number of Residues	25
Details	BINDING SITE FOR RESIDUE FAD B 472

Chain	Residue
B	TYR222
B	THR234
B	SER235
B	ARG236
B	LEU237
B	SER238
B	TRP271
B	GLU274
B	ARG278
B	TYR281
B	TRP338
B	ASN341
B	ARG344
B	LEU370
B	ASP372
B	GLY373
B	ASP374
B	ALA377
B	ASN378
B	GLY381
B	HOH511
B	HOH553
B	HOH596
B	HOH689
B	HOH797

---

site_id	AC4
Number of Residues	9
Details	BINDING SITE FOR RESIDUE MHF B 473

Chain	Residue
B	HIS44
B	GLU106
B	ASN108
B	GLU109
B	CYS292
B	LYS293
B	GLY441
B	THR443
B	HOH813

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Functional Information from PROSITE/UniProt

site_id	PS00394
Number of Residues	13
Details	DNA_PHOTOLYASES_1_1 DNA photolyases class 1 signature 1. TGyPIVDAaMRqL

Chain	Residue	Details
A	THR321-LEU333

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site_id	PS00691
Number of Residues	20
Details	DNA_PHOTOLYASES_1_2 DNA photolyases class 1 signature 2. NRlRMItASFLvKdLlidWR

Chain	Residue	Details
A	ASN341-ARG360

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:7604260, ECO:0007744|PDB:1DNP

Chain	Residue	Details
A	ASN108
A	GLU109
B	ASN108
B	GLU109

---

site_id	SWS_FT_FI2
Number of Residues	10
Details	BINDING: BINDING => ECO:0000269|PubMed:7604260

Chain	Residue	Details
A	TYR222
B	ASP372
A	THR234
A	TRP271
A	GLU274
A	ASP372
B	TYR222
B	THR234
B	TRP271
B	GLU274

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	BINDING: BINDING => ECO:0000250

Chain	Residue	Details
A	ARG226
A	GLN404
B	ARG226
B	GLN404

---

site_id	SWS_FT_FI4
Number of Residues	6
Details	SITE: Electron transfer via tryptophanyl radical

Chain	Residue	Details
A	TRP306
A	TRP359
A	TRP382
B	TRP306
B	TRP359
B	TRP382

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	3
Details	a catalytic site defined by CSA, PubMed 7604260, 9360600

Chain	Residue	Details
A	TRP306
A	TRP359
A	TRP382

---

site_id	CSA2
Number of Residues	3
Details	a catalytic site defined by CSA, PubMed 7604260, 9360600

Chain	Residue	Details
B	TRP306
B	TRP359
B	TRP382

---

site_id	MCSA1
Number of Residues	6
Details	M-CSA 183

Chain	Residue	Details
A	GLU274	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
A	TRP277	electron tunneling medium, polar/non-polar interaction, single electron acceptor, single electron donor, single electron relay, van der waals interaction
A	TRP306	single electron acceptor, single electron donor, single electron relay
A	ASN341	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor
A	TRP359	single electron acceptor, single electron donor, single electron relay
A	TRP382	single electron acceptor, single electron donor, single electron relay

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site_id	MCSA2
Number of Residues	6
Details	M-CSA 183

Chain	Residue	Details
B	GLU274	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
B	TRP277	electron tunneling medium, polar/non-polar interaction, single electron acceptor, single electron donor, single electron relay, van der waals interaction
B	TRP306	single electron acceptor, single electron donor, single electron relay
B	ASN341	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor
B	TRP359	single electron acceptor, single electron donor, single electron relay
B	TRP382	single electron acceptor, single electron donor, single electron relay

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