1DJP
CRYSTAL STRUCTURE OF PSEUDOMONAS 7A GLUTAMINASE-ASPARAGINASE WITH THE INHIBITOR DON COVALENTLY BOUND IN THE ACTIVE SITE
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004067 | molecular_function | asparaginase activity |
| A | 0004359 | molecular_function | glutaminase activity |
| A | 0006520 | biological_process | amino acid metabolic process |
| A | 0006528 | biological_process | asparagine metabolic process |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0042597 | cellular_component | periplasmic space |
| A | 0050417 | molecular_function | glutamin-(asparagin-)ase activity |
| B | 0004067 | molecular_function | asparaginase activity |
| B | 0004359 | molecular_function | glutaminase activity |
| B | 0006520 | biological_process | amino acid metabolic process |
| B | 0006528 | biological_process | asparagine metabolic process |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0042597 | cellular_component | periplasmic space |
| B | 0050417 | molecular_function | glutamin-(asparagin-)ase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE DO2 A 4000 |
| Chain | Residue |
| A | GLY1019 |
| A | SER1125 |
| B | GLU3294 |
| A | THR1020 |
| A | TYR1034 |
| A | ALA1066 |
| A | SER1067 |
| A | GLU1068 |
| A | GLY1099 |
| A | THR1100 |
| A | ASP1101 |
| site_id | AC2 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE DO2 B 4001 |
| Chain | Residue |
| A | SER1258 |
| A | GLU1294 |
| B | GLY3019 |
| B | THR3020 |
| B | TYR3034 |
| B | ALA3066 |
| B | SER3067 |
| B | GLU3068 |
| B | GLY3099 |
| B | THR3100 |
| B | ASP3101 |
| B | SER3125 |
Functional Information from PROSITE/UniProt
| site_id | PS00144 |
| Number of Residues | 9 |
| Details | ASN_GLN_ASE_1 Asparaginase / glutaminase active site signature 1. IlATGGTIA |
| Chain | Residue | Details |
| A | ILE1014-ALA1022 |
| site_id | PS00917 |
| Number of Residues | 11 |
| Details | ASN_GLN_ASE_2 Asparaginase / glutaminase active site signature 2. GiVitHGTDTL |
| Chain | Residue | Details |
| A | GLY1093-LEU1103 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 654 |
| Details | Domain: {"description":"Asparaginase/glutaminase","evidences":[{"source":"PROSITE-ProRule","id":"PRU01068","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | Active site: {"description":"Acyl-ester intermediate","evidences":[{"source":"PROSITE-ProRule","id":"PRU10099","evidenceCode":"ECO:0000255"},{"source":"PROSITE-ProRule","id":"PRU10100","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"10684596","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | Binding site: {"evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1djo |
| Chain | Residue | Details |
| A | THR1020 | |
| A | TYR1034 | |
| A | THR1100 | |
| B | GLU3294 |
| site_id | CSA2 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1djo |
| Chain | Residue | Details |
| A | GLU1294 | |
| B | THR3020 | |
| B | THR3100 | |
| B | TYR3034 |
| site_id | MCSA1 |
| Number of Residues | 6 |
| Details | M-CSA 29 |
| Chain | Residue | Details |
| A | THR1020 | covalently attached, hydrogen bond acceptor, hydrogen bond donor, nucleofuge, nucleophile, proton acceptor, proton donor |
| A | TYR1034 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, proton relay |
| A | THR1100 | hydrogen bond acceptor, hydrogen bond donor |
| A | ASP1101 | hydrogen bond acceptor |
| A | LYS1173 | hydrogen bond acceptor, hydrogen bond donor |
| A | GLU1294 | hydrogen bond acceptor, proton acceptor, proton donor, proton relay |
| site_id | MCSA2 |
| Number of Residues | 6 |
| Details | M-CSA 29 |
| Chain | Residue | Details |
| B | THR3020 | covalently attached, hydrogen bond acceptor, hydrogen bond donor, nucleofuge, nucleophile, proton acceptor, proton donor |
| B | TYR3034 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, proton relay |
| B | THR3100 | hydrogen bond acceptor, hydrogen bond donor |
| B | ASP3101 | hydrogen bond acceptor |
| B | LYS3173 | hydrogen bond acceptor, hydrogen bond donor |
| B | GLU3294 | hydrogen bond acceptor, proton acceptor, proton donor, proton relay |
