1DE7

INTERACTION OF FACTOR XIII ACTIVATION PEPTIDE WITH ALPHA-THROMBIN: CRYSTAL STRUCTURE OF THE ENZYME-SUBSTRATE COMPLEX

Functional Information from GO Data

Chain	GOid	namespace	contents
H	0004252	molecular_function	serine-type endopeptidase activity
H	0005509	molecular_function	calcium ion binding
H	0006508	biological_process	proteolysis
H	0007596	biological_process	blood coagulation
J	0004252	molecular_function	serine-type endopeptidase activity
J	0005576	cellular_component	extracellular region
J	0006508	biological_process	proteolysis
J	0007596	biological_process	blood coagulation
K	0004252	molecular_function	serine-type endopeptidase activity
K	0005509	molecular_function	calcium ion binding
K	0006508	biological_process	proteolysis
K	0007596	biological_process	blood coagulation
L	0004252	molecular_function	serine-type endopeptidase activity
L	0005576	cellular_component	extracellular region
L	0006508	biological_process	proteolysis
L	0007596	biological_process	blood coagulation

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE NA H 1

Chain	Residue
H	HOH250
H	HOH251
H	HOH252
H	ARG221
H	LYS224
H	HOH249

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site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE NA K 2

Chain	Residue
K	ARG221
K	LYS224
K	HOH249
K	HOH250
K	HOH251
K	HOH252

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site_id	AC3
Number of Residues	26
Details	BINDING SITE FOR CHAIN A OF FACTOR XIII ACTIVATION PEPTIDE (28-37)

Chain	Residue
A	HOH114
A	HOH118
A	HOH150
A	HOH193
A	HOH255
A	HOH275
A	HOH415
H	HIS57
H	TYR60
H	ARG97
H	GLU97
H	ILE174
H	ASP189
H	ALA190
H	GLY193
H	SER195
H	SER214
H	TRP215
H	GLY216
H	GLU217
H	GLY219
H	GLY226
H	HOH290
H	HOH291
H	HOH294
H	HOH331

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site_id	AC4
Number of Residues	22
Details	BINDING SITE FOR CHAIN B OF FACTOR XIII ACTIVATION PEPTIDE (28-37)

Chain	Residue
B	HOH376
K	HIS57
K	TYR60
K	TRP60
K	ARG97
K	GLU97
K	ARG173
K	ILE174
K	ASP189
K	ALA190
K	CYS191
K	GLU192
K	GLY193
K	SER195
K	SER214
K	TRP215
K	GLY216
K	GLU217
K	GLY219
K	GLY226
K	HOH253
K	HOH348

---

site_id	CAT
Number of Residues	6
Details

Chain	Residue
H	HIS57
H	ASP102
H	SER195
K	HIS57
K	ASP102
K	SER195

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Functional Information from PROSITE/UniProt

site_id	PS00134
Number of Residues	6
Details	TRYPSIN_HIS Serine proteases, trypsin family, histidine active site. LTAAHC

Chain	Residue	Details
H	LEU53-CYS58

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site_id	PS00135
Number of Residues	12
Details	TRYPSIN_SER Serine proteases, trypsin family, serine active site. DAceGDSGGPFV

Chain	Residue	Details
H	ASP189-VAL200

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	ACT_SITE: Charge relay system

Chain	Residue	Details
H	HIS57
H	ASP102
H	SER195
K	HIS57
K	ASP102
K	SER195

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site_id	SWS_FT_FI2
Number of Residues	2
Details	CARBOHYD: N-linked (GlcNAc...) (complex) asparagine => ECO:0000269|PubMed:16335952, ECO:0000269|PubMed:19139490, ECO:0000269|PubMed:19159218, ECO:0000269|PubMed:19838169, ECO:0000269|PubMed:873923

Chain	Residue	Details
H	ASN60
K	ASN60

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