1CZF

ENDO-POLYGALACTURONASE II FROM ASPERGILLUS NIGER

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004650	molecular_function	polygalacturonase activity
A	0005975	biological_process	carbohydrate metabolic process
B	0004650	molecular_function	polygalacturonase activity
B	0005975	biological_process	carbohydrate metabolic process

Functional Information from PROSITE/UniProt

site_id	PS00502
Number of Residues	14
Details	POLYGALACTURONASE Polygalacturonase active site. GgtCigGHGLs.IGS

Chain	Residue	Details
A	GLY216-SER229

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: Proton donor => ECO:0000305|PubMed:10521427

Chain	Residue	Details
A	ASP201
B	ASP201

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site_id	SWS_FT_FI2
Number of Residues	2
Details	ACT_SITE: ACT_SITE => ECO:0000305|PubMed:10521427

Chain	Residue	Details
A	HIS223
B	HIS223

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site_id	SWS_FT_FI3
Number of Residues	2
Details	CARBOHYD: N-linked (GlcNAc...) (high mannose) asparagine => ECO:0000269|PubMed:10521427, ECO:0000269|PubMed:9276972

Chain	Residue	Details
A	ASN240
B	ASN240

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Catalytic Information from CSA

site_id	CSA1
Number of Residues	4
Details	a catalytic site defined by CSA, PubMed 10521427, 11518536

Chain	Residue	Details
A	ASP202
A	ASP180
A	ASP201
A	HIS223

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site_id	MCSA1
Number of Residues	6
Details	M-CSA 480

Chain	Residue	Details
A	ASP180	proton shuttle (general acid/base)
A	ASP201	proton shuttle (general acid/base)
A	ASP202	electrostatic stabiliser
A	HIS223	proton shuttle (general acid/base)
A	ARG256	modifies pKa
A	LYS258	modifies pKa

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site_id	MCSA2
Number of Residues	6
Details	M-CSA 480

Chain	Residue	Details
B	ASP180	proton shuttle (general acid/base)
B	ASP201	proton shuttle (general acid/base)
B	ASP202	electrostatic stabiliser
B	HIS223	proton shuttle (general acid/base)
B	ARG256	modifies pKa
B	LYS258	modifies pKa

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