1CZF
ENDO-POLYGALACTURONASE II FROM ASPERGILLUS NIGER
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-01-17 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 65.497, 201.241, 49.065 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.680 |
| R-factor | 0.166 |
| Rwork | 0.166 |
| R-free | 0.19100 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.654 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.700 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.035 | 0.078 * |
| Total number of observations | 263929 * | |
| Number of reflections | 69332 | |
| <I/σ(I)> | 13.22 | |
| Completeness [%] | 92.0 | 64.9 |
| Redundancy | 3.8 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | PEG 4000, zinc sulphate, sodium acetate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium acetate | 100 (mM) | |
| 2 | 1 | reservoir | 100 (mM) | ||
| 3 | 1 | reservoir | PEG8000 | 11-13 (%(w/v)) | |
| 4 | 1 | drop | protein | 9 (mg/ml) | |
| 5 | 1 | drop | sodium acetate | 10 (mM) |
