1CS4
COMPLEX OF GS-ALPHA WITH THE CATALYTIC DOMAINS OF MAMMALIAN ADENYLYL CYCLASE: COMPLEX WITH 2'-DEOXY-ADENOSINE 3'-MONOPHOSPHATE, PYROPHOSPHATE AND MG
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0009190 | biological_process | cyclic nucleotide biosynthetic process |
| A | 0016849 | molecular_function | phosphorus-oxygen lyase activity |
| A | 0035556 | biological_process | intracellular signal transduction |
| B | 0009190 | biological_process | cyclic nucleotide biosynthetic process |
| B | 0016849 | molecular_function | phosphorus-oxygen lyase activity |
| B | 0035556 | biological_process | intracellular signal transduction |
| C | 0000166 | molecular_function | nucleotide binding |
| C | 0003924 | molecular_function | GTPase activity |
| C | 0005159 | molecular_function | insulin-like growth factor receptor binding |
| C | 0005525 | molecular_function | GTP binding |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005834 | cellular_component | heterotrimeric G-protein complex |
| C | 0005886 | cellular_component | plasma membrane |
| C | 0007165 | biological_process | signal transduction |
| C | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| C | 0007189 | biological_process | adenylate cyclase-activating G protein-coupled receptor signaling pathway |
| C | 0007191 | biological_process | adenylate cyclase-activating dopamine receptor signaling pathway |
| C | 0007606 | biological_process | sensory perception of chemical stimulus |
| C | 0010856 | molecular_function | adenylate cyclase activator activity |
| C | 0016020 | cellular_component | membrane |
| C | 0016787 | molecular_function | hydrolase activity |
| C | 0019001 | molecular_function | guanyl nucleotide binding |
| C | 0031683 | molecular_function | G-protein beta/gamma-subunit complex binding |
| C | 0031698 | molecular_function | beta-2 adrenergic receptor binding |
| C | 0031748 | molecular_function | D1 dopamine receptor binding |
| C | 0031852 | molecular_function | mu-type opioid receptor binding |
| C | 0035255 | molecular_function | ionotropic glutamate receptor binding |
| C | 0046872 | molecular_function | metal ion binding |
| C | 0051430 | molecular_function | corticotropin-releasing hormone receptor 1 binding |
| C | 0071880 | biological_process | adenylate cyclase-activating adrenergic receptor signaling pathway |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG C 396 |
| Chain | Residue |
| C | SER54 |
| C | THR204 |
| C | GSP1000 |
| C | HOH1007 |
| C | HOH1010 |
| site_id | AC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG A 581 |
| Chain | Residue |
| B | 1011082 |
| A | ASP396 |
| A | ILE397 |
| A | ASP440 |
| A | POP1005 |
| site_id | AC3 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE CL C 398 |
| Chain | Residue |
| C | ALA249 |
| site_id | AC4 |
| Number of Residues | 22 |
| Details | BINDING SITE FOR RESIDUE GSP C 1000 |
| Chain | Residue |
| C | GLY49 |
| C | GLU50 |
| C | SER51 |
| C | GLY52 |
| C | LYS53 |
| C | SER54 |
| C | THR55 |
| C | ASP173 |
| C | LEU198 |
| C | ARG199 |
| C | ARG201 |
| C | THR204 |
| C | GLY226 |
| C | ASN292 |
| C | LYS293 |
| C | ASP295 |
| C | CYS365 |
| C | ALA366 |
| C | VAL367 |
| C | MG396 |
| C | HOH1007 |
| C | HOH1010 |
| site_id | AC5 |
| Number of Residues | 12 |
| Details | BINDING SITE FOR RESIDUE 101 B 1082 |
| Chain | Residue |
| A | ASP396 |
| A | PHE400 |
| A | THR401 |
| A | LEU438 |
| A | ASP440 |
| A | MG581 |
| A | POP1005 |
| B | LYS938 |
| B | ASP1018 |
| B | ILE1019 |
| B | ASN1025 |
| B | SER1028 |
| site_id | AC6 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE FOK A 1002 |
| Chain | Residue |
| A | VAL506 |
| A | TRP507 |
| A | SER508 |
| A | VAL511 |
| A | THR512 |
| B | HOH17 |
| B | PHE895 |
| B | LYS896 |
| B | GLY941 |
| B | SER942 |
| site_id | AC7 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MES A 1003 |
| Chain | Residue |
| A | TYR553 |
| A | GLU556 |
| B | LYS1067 |
| B | GLY1068 |
| B | ASP1069 |
| B | LEU1070 |
| site_id | AC8 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MES B 1083 |
| Chain | Residue |
| B | LYS1038 |
| B | LYS1038 |
| B | PHE1074 |
| B | VAL1075 |
| B | ASN1076 |
| B | THR1077 |
| site_id | AC9 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE POP A 1005 |
| Chain | Residue |
| A | HOH11 |
| A | ASP396 |
| A | ILE397 |
| A | GLY399 |
| A | PHE400 |
| A | THR401 |
| A | ARG484 |
| A | MG581 |
| B | ARG1029 |
| B | LYS1065 |
| B | 1011082 |
Functional Information from PROSITE/UniProt
| site_id | PS00452 |
| Number of Residues | 24 |
| Details | GUANYLATE_CYCLASE_1 Guanylate cyclase signature. GVI.GaqkpqYdIWGNTVNvasrmD |
| Chain | Residue | Details |
| B | GLY1008-ASP1031 | |
| A | GLY495-GLU518 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 5 |
| Details | BINDING: BINDING => ECO:0000305|PubMed:10427002, ECO:0000305|PubMed:11087399, ECO:0000305|PubMed:15591060, ECO:0000305|PubMed:16766715, ECO:0000305|PubMed:19243146, ECO:0000305|PubMed:9395396, ECO:0000305|PubMed:9417641 |
| Chain | Residue | Details |
| C | GLY47 | |
| C | LEU197 | |
| C | ASP223 | |
| C | ASN292 | |
| C | ALA366 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:10427002, ECO:0000269|PubMed:11087399, ECO:0000269|PubMed:15591060, ECO:0000269|PubMed:19243146, ECO:0000269|PubMed:9395396, ECO:0000269|PubMed:9417641, ECO:0000305|PubMed:16766715 |
| Chain | Residue | Details |
| C | SER54 | |
| C | THR204 | |
| A | GLY521 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P63092 |
| Chain | Residue | Details |
| C | SER352 | |
| A | LEU565 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | LIPID: N-palmitoyl glycine => ECO:0000269|PubMed:12574119 |
| Chain | Residue | Details |
| C | GLY2 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | LIPID: S-palmitoyl cysteine => ECO:0000269|PubMed:12574119 |
| Chain | Residue | Details |
| C | CYS3 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000250|UniProtKB:P63092 |
| Chain | Residue | Details |
| C | LYS300 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1ab8 |
| Chain | Residue | Details |
| B | ARG1029 |
| site_id | CSA2 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1ab8 |
| Chain | Residue | Details |
| A | HIS516 |
| site_id | CSA3 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1ab8 |
| Chain | Residue | Details |
| C | THR204 | |
| C | ARG201 | |
| C | GLU50 | |
| C | GLN227 |
| site_id | CSA4 |
| Number of Residues | 1 |
| Details | Annotated By Reference To The Literature 1ab8 |
| Chain | Residue | Details |
| C | GLN227 |
| site_id | MCSA1 |
| Number of Residues | 2 |
| Details | M-CSA 58 |
| Chain | Residue | Details |
| B | ARG1029 | electrostatic stabiliser |
| B | LYS1065 | electrostatic stabiliser |
| A | ASP440 | metal ligand |
