Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

1CS4

COMPLEX OF GS-ALPHA WITH THE CATALYTIC DOMAINS OF MAMMALIAN ADENYLYL CYCLASE: COMPLEX WITH 2'-DEOXY-ADENOSINE 3'-MONOPHOSPHATE, PYROPHOSPHATE AND MG

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0009190	biological_process	cyclic nucleotide biosynthetic process
A	0016849	molecular_function	phosphorus-oxygen lyase activity
A	0035556	biological_process	intracellular signal transduction
B	0009190	biological_process	cyclic nucleotide biosynthetic process
B	0016849	molecular_function	phosphorus-oxygen lyase activity
B	0035556	biological_process	intracellular signal transduction
C	0000166	molecular_function	nucleotide binding
C	0003924	molecular_function	GTPase activity
C	0005159	molecular_function	insulin-like growth factor receptor binding
C	0005525	molecular_function	GTP binding
C	0005737	cellular_component	cytoplasm
C	0005834	cellular_component	heterotrimeric G-protein complex
C	0005886	cellular_component	plasma membrane
C	0007165	biological_process	signal transduction
C	0007186	biological_process	G protein-coupled receptor signaling pathway
C	0007189	biological_process	adenylate cyclase-activating G protein-coupled receptor signaling pathway
C	0007191	biological_process	adenylate cyclase-activating dopamine receptor signaling pathway
C	0007606	biological_process	sensory perception of chemical stimulus
C	0010856	molecular_function	adenylate cyclase activator activity
C	0016787	molecular_function	hydrolase activity
C	0019001	molecular_function	guanyl nucleotide binding
C	0031683	molecular_function	G-protein beta/gamma-subunit complex binding
C	0031698	molecular_function	beta-2 adrenergic receptor binding
C	0031748	molecular_function	D1 dopamine receptor binding
C	0031852	molecular_function	mu-type opioid receptor binding
C	0035255	molecular_function	ionotropic glutamate receptor binding
C	0046872	molecular_function	metal ion binding
C	0051430	molecular_function	corticotropin-releasing hormone receptor 1 binding
C	0071880	biological_process	adenylate cyclase-activating adrenergic receptor signaling pathway

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG C 396

Chain	Residue
C	SER54
C	THR204
C	GSP1000
C	HOH1007
C	HOH1010

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG A 581

Chain	Residue
B	1011082
A	ASP396
A	ILE397
A	ASP440
A	POP1005

site_id	AC3
Number of Residues	1
Details	BINDING SITE FOR RESIDUE CL C 398

Chain	Residue
C	ALA249

site_id	AC4
Number of Residues	22
Details	BINDING SITE FOR RESIDUE GSP C 1000

Chain	Residue
C	GLY49
C	GLU50
C	SER51
C	GLY52
C	LYS53
C	SER54
C	THR55
C	ASP173
C	LEU198
C	ARG199
C	ARG201
C	THR204
C	GLY226
C	ASN292
C	LYS293
C	ASP295
C	CYS365
C	ALA366
C	VAL367
C	MG396
C	HOH1007
C	HOH1010

site_id	AC5
Number of Residues	12
Details	BINDING SITE FOR RESIDUE 101 B 1082

Chain	Residue
A	ASP396
A	PHE400
A	THR401
A	LEU438
A	ASP440
A	MG581
A	POP1005
B	LYS938
B	ASP1018
B	ILE1019
B	ASN1025
B	SER1028

site_id	AC6
Number of Residues	10
Details	BINDING SITE FOR RESIDUE FOK A 1002

Chain	Residue
A	VAL506
A	TRP507
A	SER508
A	VAL511
A	THR512
B	HOH17
B	PHE895
B	LYS896
B	GLY941
B	SER942

site_id	AC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MES A 1003

Chain	Residue
A	TYR553
A	GLU556
B	LYS1067
B	GLY1068
B	ASP1069
B	LEU1070

site_id	AC8
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MES B 1083

Chain	Residue
B	LYS1038
B	LYS1038
B	PHE1074
B	VAL1075
B	ASN1076
B	THR1077

site_id	AC9
Number of Residues	11
Details	BINDING SITE FOR RESIDUE POP A 1005

Chain	Residue
A	HOH11
A	ASP396
A	ILE397
A	GLY399
A	PHE400
A	THR401
A	ARG484
A	MG581
B	ARG1029
B	LYS1065
B	1011082

Functional Information from PROSITE/UniProt

site_id	PS00452
Number of Residues	24
Details	GUANYLATE_CYCLASE_1 Guanylate cyclase signature. GVI.GaqkpqYdIWGNTVNvasrmD

Chain	Residue	Details
B	GLY1008-ASP1031
A	GLY495-GLU518

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	127
Details	Domain: {"description":"Guanylate cyclase 1","evidences":[{"source":"PROSITE-ProRule","id":"PRU00099","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"PubMed","id":"10427002","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"11087399","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"16766715","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"19243146","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	3
Details	Binding site: {"evidences":[{"source":"PubMed","id":"10427002","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	20
Details	Region: {"description":"Interaction with GNAS","evidences":[{"source":"PubMed","id":"11087399","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"10427002","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"11087399","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"15591060","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"16766715","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"19243146","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"10427002","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1CJK","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3MAA","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	13
Details	Region: {"description":"G1 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	8
Details	Region: {"description":"G2 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI9
Number of Residues	9
Details	Region: {"description":"G3 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI10
Number of Residues	7
Details	Region: {"description":"G4 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI11
Number of Residues	5
Details	Region: {"description":"G5 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI12
Number of Residues	23
Details	Binding site: {"evidences":[{"source":"PubMed","id":"10427002","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"11087399","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"15591060","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"16766715","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"19243146","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"9395396","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"9417641","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI13
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"10427002","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"11087399","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15591060","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19243146","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9395396","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9417641","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16766715","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI14
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P63092","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI15
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"UniProtKB","id":"P63092","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

Catalytic Information from CSA

site_id	CSA1
Number of Residues	1
Details	Annotated By Reference To The Literature 1ab8

Chain	Residue	Details
B	ARG1029

site_id	CSA2
Number of Residues	1
Details	Annotated By Reference To The Literature 1ab8

Chain	Residue	Details
A	HIS516

site_id	CSA3
Number of Residues	4
Details	Annotated By Reference To The Literature 1ab8

Chain	Residue	Details
C	THR204
C	ARG201
C	GLU50
C	GLN227

site_id	CSA4
Number of Residues	1
Details	Annotated By Reference To The Literature 1ab8

Chain	Residue	Details
C	GLN227

site_id	MCSA1
Number of Residues	2
Details	M-CSA 58

Chain	Residue	Details
B	ARG1029	electrostatic stabiliser
B	LYS1065	electrostatic stabiliser
A	ASP440	metal ligand

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)