1CS1

CYSTATHIONINE GAMMA-SYNTHASE (CGS) FROM ESCHERICHIA COLI

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000096	biological_process	sulfur amino acid metabolic process
A	0003962	molecular_function	cystathionine gamma-synthase activity
A	0004123	molecular_function	cystathionine gamma-lyase activity
A	0005737	cellular_component	cytoplasm
A	0008652	biological_process	amino acid biosynthetic process
A	0009086	biological_process	methionine biosynthetic process
A	0016740	molecular_function	transferase activity
A	0019343	biological_process	cysteine biosynthetic process via cystathionine
A	0019346	biological_process	transsulfuration
A	0030170	molecular_function	pyridoxal phosphate binding
B	0000096	biological_process	sulfur amino acid metabolic process
B	0003962	molecular_function	cystathionine gamma-synthase activity
B	0004123	molecular_function	cystathionine gamma-lyase activity
B	0005737	cellular_component	cytoplasm
B	0008652	biological_process	amino acid biosynthetic process
B	0009086	biological_process	methionine biosynthetic process
B	0016740	molecular_function	transferase activity
B	0019343	biological_process	cysteine biosynthetic process via cystathionine
B	0019346	biological_process	transsulfuration
B	0030170	molecular_function	pyridoxal phosphate binding
C	0000096	biological_process	sulfur amino acid metabolic process
C	0003962	molecular_function	cystathionine gamma-synthase activity
C	0004123	molecular_function	cystathionine gamma-lyase activity
C	0005737	cellular_component	cytoplasm
C	0008652	biological_process	amino acid biosynthetic process
C	0009086	biological_process	methionine biosynthetic process
C	0016740	molecular_function	transferase activity
C	0019343	biological_process	cysteine biosynthetic process via cystathionine
C	0019346	biological_process	transsulfuration
C	0030170	molecular_function	pyridoxal phosphate binding
D	0000096	biological_process	sulfur amino acid metabolic process
D	0003962	molecular_function	cystathionine gamma-synthase activity
D	0004123	molecular_function	cystathionine gamma-lyase activity
D	0005737	cellular_component	cytoplasm
D	0008652	biological_process	amino acid biosynthetic process
D	0009086	biological_process	methionine biosynthetic process
D	0016740	molecular_function	transferase activity
D	0019343	biological_process	cysteine biosynthetic process via cystathionine
D	0019346	biological_process	transsulfuration
D	0030170	molecular_function	pyridoxal phosphate binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	8
Details	BINDING SITE FOR RESIDUE DHD D 1501

Chain	Residue
D	HOH1654
D	HOH1664
D	HOH1664
D	ALA157
D	ALA157
D	LYS158
D	LYS158
D	HIS161

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site_id	PLA
Number of Residues	1
Details	COFACTOR SITE CHAIN A

Chain	Residue
A	LLP198

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site_id	PLB
Number of Residues	1
Details	COFACTOR SITE CHAIN B

Chain	Residue
B	LLP198

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site_id	PLC
Number of Residues	1
Details	COFACTOR SITE CHAIN C

Chain	Residue
C	LLP198

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site_id	PLD
Number of Residues	1
Details	COFACTOR SITE CHAIN D

Chain	Residue
D	LLP198

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Functional Information from PROSITE/UniProt

site_id	PS00868
Number of Residues	15
Details	CYS_MET_METAB_PP Cys/Met metabolism enzymes pyridoxal-phosphate attachment site. DLvlhSCTKYLnGHS

Chain	Residue	Details
A	ASP190-SER204

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	Modified residue: {"description":"N6-(pyridoxal phosphate)lysine","evidences":[{"source":"PubMed","id":"3307782","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

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Catalytic Information from CSA

site_id	CSA1
Number of Residues	4
Details	a catalytic site defined by CSA, PubMed 9843488, 8831789, 14503880

Chain	Residue	Details
A	LLP198
A	TYR101
A	ASP173
C	ARG48

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site_id	CSA2
Number of Residues	3
Details	a catalytic site defined by CSA, PubMed 9843488, 8831789, 14503880

Chain	Residue	Details
A	ARG48
C	ASP173
C	TYR101

---

site_id	CSA3
Number of Residues	3
Details	a catalytic site defined by CSA, PubMed 9843488, 8831789, 14503880

Chain	Residue	Details
D	ASP173
D	TYR101
B	ARG48

---

site_id	CSA4
Number of Residues	3
Details	a catalytic site defined by CSA, PubMed 9843488, 8831789, 14503880

Chain	Residue	Details
D	ARG48
B	TYR101
B	ASP173

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site_id	MCSA1
Number of Residues	4
Details	M-CSA 759

Chain	Residue	Details
A	ARG48	electrostatic stabiliser
A	TYR101	electrostatic stabiliser, proton acceptor, proton donor
A	ASP173	electrostatic stabiliser
A	LLP198	covalently attached, electron pair acceptor, electron pair donor, electrostatic stabiliser, nucleofuge, nucleophile, proton acceptor, proton donor

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site_id	MCSA2
Number of Residues	4
Details	M-CSA 759

Chain	Residue	Details
B	ARG48	electrostatic stabiliser
B	TYR101	electrostatic stabiliser, proton acceptor, proton donor
B	ASP173	electrostatic stabiliser
B	LLP198	covalently attached, electron pair acceptor, electron pair donor, electrostatic stabiliser, nucleofuge, nucleophile, proton acceptor, proton donor

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site_id	MCSA3
Number of Residues	4
Details	M-CSA 759

Chain	Residue	Details
C	ARG48	electrostatic stabiliser
C	TYR101	electrostatic stabiliser, proton acceptor, proton donor
C	ASP173	electrostatic stabiliser
C	LLP198	covalently attached, electron pair acceptor, electron pair donor, electrostatic stabiliser, nucleofuge, nucleophile, proton acceptor, proton donor

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site_id	MCSA4
Number of Residues	4
Details	M-CSA 759

Chain	Residue	Details
D	ARG48	electrostatic stabiliser
D	TYR101	electrostatic stabiliser, proton acceptor, proton donor
D	ASP173	electrostatic stabiliser
D	LLP198	covalently attached, electron pair acceptor, electron pair donor, electrostatic stabiliser, nucleofuge, nucleophile, proton acceptor, proton donor

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