1CS1
CYSTATHIONINE GAMMA-SYNTHASE (CGS) FROM ESCHERICHIA COLI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-09-15 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 160.040, 61.300, 153.840 |
Unit cell angles | 90.00, 104.18, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.500 |
R-factor | 0.2 |
Rwork | 0.200 |
R-free | 0.25500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cl1 |
RMSD bond length | 0.009 |
RMSD bond angle | 25.020 * |
Data reduction software | DENZO |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE ((STAND ALONE)) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 8.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.066 | 0.205 |
Number of reflections | 200884 | 16087 * |
<I/σ(I)> | 23.4 | 7.2 |
Completeness [%] | 86.9 | 70.1 |
Redundancy | 2.7 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7 * | Wahl, M.C., (1997) FEBS Lett., 414, 492. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | HEPES-NaOH | 33 (mM) | |
3 | 1 | drop | PEG4000 | 6.7 (%(w/v)) | |
4 | 1 | drop | 2-propanol | 3.3 (%(v/v)) | |
5 | 1 | reservoir | HEPES-NaOH | 100 (mM) | |
6 | 1 | reservoir | PEG4000 | 20 (%(w/v)) | |
7 | 1 | reservoir | 2-propanol | 10 (%(v/v)) |