1CBG

THE CRYSTAL STRUCTURE OF A CYANOGENIC BETA-GLUCOSIDASE FROM WHITE CLOVER (TRIFOLIUM REPENS L.), A FAMILY 1 GLYCOSYL-HYDROLASE

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
A	0005975	biological_process	carbohydrate metabolic process
A	0008152	biological_process	metabolic process
A	0008422	molecular_function	beta-glucosidase activity
A	0016787	molecular_function	hydrolase activity
A	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
A	0102483	molecular_function	scopolin beta-glucosidase activity

Functional Information from PROSITE/UniProt

site_id	PS00572
Number of Residues	9
Details	GLYCOSYL_HYDROL_F1_1 Glycosyl hydrolases family 1 active site. IYITENGRN

Chain	Residue	Details
A	ILE393-ASN401

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site_id	PS00653
Number of Residues	15
Details	GLYCOSYL_HYDROL_F1_2 Glycosyl hydrolases family 1 N-terminal signature. FvFGtAsSAFQyEgA

Chain	Residue	Details
A	PHE23-ALA37

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Proton donor => ECO:0000250|UniProtKB:Q7XSK0

Chain	Residue	Details
A	GLU183

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site_id	SWS_FT_FI2
Number of Residues	1
Details	ACT_SITE: Nucleophile => ECO:0000250|UniProtKB:Q7XSK0

Chain	Residue	Details
A	GLU397

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site_id	SWS_FT_FI3
Number of Residues	3
Details	BINDING: BINDING => ECO:0000250|UniProtKB:Q8L7J2

Chain	Residue	Details
A	GLN33
A	HIS137
A	TYR326

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site_id	SWS_FT_FI4
Number of Residues	1
Details	BINDING: BINDING => ECO:0000250|UniProtKB:Q8GU20

Chain	Residue	Details
A	ASN182

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site_id	SWS_FT_FI5
Number of Residues	1
Details	BINDING: BINDING => ECO:0000250|UniProtKB:Q9SPP9

Chain	Residue	Details
A	GLU397

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site_id	SWS_FT_FI6
Number of Residues	2
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255|PROSITE-ProRule:PRU00498

Chain	Residue	Details
A	ASN209
A	ASN401

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	6
Details	M-CSA 442

Chain	Residue	Details
A	ARG91	modifies pKa
A	HIS137	transition state stabiliser
A	GLU183	proton shuttle (general acid/base)
A	ASN324	modifies pKa, steric role
A	TYR326	modifies pKa
A	GLU397	covalent catalysis

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