1CBG
THE CRYSTAL STRUCTURE OF A CYANOGENIC BETA-GLUCOSIDASE FROM WHITE CLOVER (TRIFOLIUM REPENS L.), A FAMILY 1 GLYCOSYL-HYDROLASE
Experimental procedure
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1994-04-16 |
Detector | MARRESEARCH |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 70.326, 70.326, 249.096 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 9.000 - 2.150 |
R-factor | 0.189 * |
Rwork | 0.195 |
R-free | 0.24700 |
RMSD bond length | 0.010 * |
RMSD bond angle | 0.036 * |
Data reduction software | DENZO |
Phasing software | X-PLOR (3.1) |
Refinement software | PROLSQ |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 15.000 |
High resolution limit [Å] | 2.150 |
Rmerge | 0.057 |
Number of reflections | 30663 |
Completeness [%] | 88.0 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.2 * | 20 * | Tolley, S.P., (1993) J.Mol.Biol., 229, 791. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulphate | 48-52 (%(w/v)) | |
2 | 1 | reservoir | Tris-maleate | 200 (mM) |