1CB2

CELLOBIOHYDROLASE II, CATALYTIC DOMAIN, MUTANT Y169F

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
A	0005975	biological_process	carbohydrate metabolic process
A	0030245	biological_process	cellulose catabolic process
B	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
B	0005975	biological_process	carbohydrate metabolic process
B	0030245	biological_process	cellulose catabolic process

Functional Information from PDB Data

site_id	ST1
Number of Residues	3
Details	CATALYTIC SITE INCLUDING MUTATED TYR-> PHE.

Chain	Residue
A	ASP175
A	ASP221
A	PHE169

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site_id	ST2
Number of Residues	3
Details	CATALYTIC SITE INCLUDING MUTATED TYR-> PHE.

Chain	Residue
B	ASP175
B	ASP221
B	PHE169

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Functional Information from PROSITE/UniProt

site_id	PS00656
Number of Residues	10
Details	GLYCOSYL_HYDROL_F6_2 Glycosyl hydrolases family 6 signature 2. LLVIEPDSLA

Chain	Residue	Details
A	LEU215-ALA224

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	Active site: {"description":"Proton donor","evidences":[{"source":"PubMed","id":"12188666","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI2
Number of Residues	2
Details	Site: {"description":"Transition state stabilizer that also modulates the pKa of Asp-245 and may act as a proton acceptor through a water chain","evidences":[{"source":"PubMed","id":"2377893","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI3
Number of Residues	6
Details	Glycosylation: {"description":"O-linked (Man...) threonine","evidences":[{"source":"PubMed","id":"12188666","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8875646","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI4
Number of Residues	8
Details	Glycosylation: {"description":"O-linked (Man...) serine","evidences":[{"source":"PubMed","id":"12188666","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8875646","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI5
Number of Residues	2
Details	Glycosylation: {"description":"N-linked (GlcNAc) asparagine","evidences":[{"source":"PubMed","id":"12188666","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8875646","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI6
Number of Residues	2
Details	Glycosylation: {"description":"N-linked (GlcNAc...) (high mannose) asparagine","evidences":[{"source":"PubMed","id":"12188666","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8875646","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

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Catalytic Information from CSA

site_id	CSA1
Number of Residues	4
Details	Annotated By Reference To The Literature 1qk2

Chain	Residue	Details
A	ASP175
A	ASP221
A	PHE169
A	ARG174

---

site_id	CSA2
Number of Residues	4
Details	Annotated By Reference To The Literature 1qk2

Chain	Residue	Details
B	ASP175
B	ASP221
B	PHE169
B	ARG174

---

site_id	CSA3
Number of Residues	2
Details	Annotated By Reference To The Literature 1qk2

Chain	Residue	Details
A	ASP221
A	ASP401

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site_id	CSA4
Number of Residues	2
Details	Annotated By Reference To The Literature 1qk2

Chain	Residue	Details
B	ASP221
B	ASP401

---

site_id	MCSA1
Number of Residues	6
Details	M-CSA 440

Chain	Residue	Details
A	PHE169	modifies pKa, steric role
A	ARG174	electrostatic stabiliser
A	ASP175	modifies pKa, proton shuttle (general acid/base), transition state stabiliser
A	SER181	electrostatic stabiliser
A	ASP221	proton shuttle (general acid/base)
A	ASP401	electrostatic stabiliser

---

site_id	MCSA2
Number of Residues	6
Details	M-CSA 440

Chain	Residue	Details
B	PHE169	modifies pKa, steric role
B	ARG174	electrostatic stabiliser
B	ASP175	modifies pKa, proton shuttle (general acid/base), transition state stabiliser
B	SER181	electrostatic stabiliser
B	ASP221	proton shuttle (general acid/base)
B	ASP401	electrostatic stabiliser

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