1CB2

CELLOBIOHYDROLASE II, CATALYTIC DOMAIN, MUTANT Y169F

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1993-04-02
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 1 21 1
Unit cell lengths	49.100, 75.800, 92.900
Unit cell angles	90.00, 103.20, 90.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.21
Rwork	0.210
R-free	0.23200
RMSD bond length	0.005
RMSD bond angle	24.000 *
Data reduction software	HAMLIN
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.800 *
Rmerge	0.068
Total number of observations	202105 *
Number of reflections	46849
Completeness [%]	91.7
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	PEG6000	10 (%)
3	1	drop	MES	20 (mM)
4	1	reservoir	PEG6000	20 (%)
5	1	reservoir	MES	20 (mM)