1BWL

OLD YELLOW ENZYME (OYE1) DOUBLE MUTANT H191N:N194H

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003959	molecular_function	NADPH dehydrogenase activity
A	0006915	biological_process	apoptotic process
A	0010181	molecular_function	FMN binding
A	0016491	molecular_function	oxidoreductase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	19
Details	BINDING SITE FOR RESIDUE FMN A 401

Chain	Residue
A	PRO34
A	ALA323
A	GLY324
A	ASN325
A	TYR346
A	GLY347
A	ARG348
A	TYR375
A	HOH536
A	HOH540
A	HOH615
A	PRO35
A	LEU36
A	THR37
A	GLY72
A	GLN114
A	ASN191
A	HIS194
A	ARG243

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	Active site: {"description":"Proton donor","evidences":[{"source":"PubMed","id":"9830020","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI2
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11668181","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"7881908","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9830019","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI3
Number of Residues	3
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11668181","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"7881908","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

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Catalytic Information from CSA

site_id	CSA1
Number of Residues	4
Details	a catalytic site defined by CSA, PubMed 9830019, 11668181, 7881908, 10097075, 9830020

Chain	Residue	Details
A	ASN191
A	THR37
A	HIS194
A	TYR196

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site_id	MCSA1
Number of Residues	5
Details	M-CSA 319

Chain	Residue	Details
A	THR37	electrostatic stabiliser, hydrogen bond donor
A	ASN191	electrostatic stabiliser, hydrogen bond donor
A	HIS194	electrostatic stabiliser, hydrogen bond donor
A	TYR196	electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor
A	ASN251	hydrogen bond donor, increase acidity

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