1BWL
OLD YELLOW ENZYME (OYE1) DOUBLE MUTANT H191N:N194H
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Detector | SDMS |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 143.600, 143.600, 42.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.700 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.27300 |
| Structure solution method | OTHER |
| Starting model (for MR) | 1oya |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.600 |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.117 * | 0.303 * |
| Total number of observations | 42885 * | |
| Number of reflections | 11781 | |
| <I/σ(I)> | 11.1 | 3.3 |
| Completeness [%] | 91.4 | 82.1 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 4 * | Fox, K.M., (1993) J. Mol. Biol., 234, 502. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 40 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | drop | phrnylmethylsulfonyl fluoride | 0.01 (M) | |
| 5 | 1 | reservoir | HEPES | 0.1 (M) | |
| 6 | 1 | reservoir | PEG400 | 35 (%(w/v)) | |
| 7 | 1 | reservoir | 0.2 (M) |
