1BF2

STRUCTURE OF PSEUDOMONAS ISOAMYLASE

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
A	0005576	cellular_component	extracellular region
A	0005975	biological_process	carbohydrate metabolic process
A	0016787	molecular_function	hydrolase activity
A	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
A	0019156	molecular_function	isoamylase activity
A	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA A 751

Chain	Residue
A	ASP128
A	GLU229
A	THR230
A	ASN232
A	ASP259
A	HOH1002

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	Active site: {"description":"Nucleophile"}

Chain

Residue

Details

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site_id	SWS_FT_FI2
Number of Residues	1
Details	Active site: {"description":"Proton donor"}

Chain

Residue

Details

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site_id	SWS_FT_FI3
Number of Residues	5
Details	Binding site: {"evidences":[{"source":"PubMed","id":"9719642","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1BF2","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI4
Number of Residues	1
Details	Site: {"description":"Transition state stabilizer","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

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Catalytic Information from CSA

site_id	CSA1
Number of Residues	3
Details	a catalytic site defined by CSA, PubMed 9719642, 12196524

Chain	Residue	Details
A	ASP510
A	ASP375
A	GLU435

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site_id	MCSA1
Number of Residues	8
Details	M-CSA 421

Chain	Residue	Details
A	ASP128	metal ligand
A	GLU229	metal ligand
A	THR230	metal ligand
A	ASN232	metal ligand
A	ASP259	metal ligand
A	ASP375	covalent catalysis
A	GLU435	proton shuttle (general acid/base)
A	ASP510	transition state stabiliser

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