1BF2

STRUCTURE OF PSEUDOMONAS ISOAMYLASE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1994-05-24
Detector	RIGAKU
Spacegroup name	P 21 21 21
Unit cell lengths	138.900, 152.400, 53.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.161
Rwork	0.161
R-free	0.21400
RMSD bond length	0.009 *
RMSD bond angle	1.769 *
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	17.000
High resolution limit [Å]	2.200
Rmerge	0.078
Total number of observations	186961 *
Number of reflections	57176
Completeness [%]	97.1
Redundancy	3.27

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	4.8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	20 (mg/ml)
2	1	2	ammonium sulfate	0.6 (M)	pH4.8