1BC3

RECOMBINANT RAT ANNEXIN V, TRIPLE MUTANT (T72K, S144K, S228K)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001786	molecular_function	phosphatidylserine binding
A	0005388	molecular_function	P-type calcium transporter activity
A	0005509	molecular_function	calcium ion binding
A	0005515	molecular_function	protein binding
A	0005544	molecular_function	calcium-dependent phospholipid binding
A	0005615	cellular_component	extracellular space
A	0005737	cellular_component	cytoplasm
A	0007596	biological_process	blood coagulation
A	0007599	biological_process	hemostasis
A	0008021	cellular_component	synaptic vesicle
A	0009897	cellular_component	external side of plasma membrane
A	0012506	cellular_component	vesicle membrane
A	0014704	cellular_component	intercalated disc
A	0017046	molecular_function	peptide hormone binding
A	0030018	cellular_component	Z disc
A	0030195	biological_process	negative regulation of blood coagulation
A	0030425	cellular_component	dendrite
A	0030672	cellular_component	synaptic vesicle membrane
A	0030971	molecular_function	receptor tyrosine kinase binding
A	0042383	cellular_component	sarcolemma
A	0042802	molecular_function	identical protein binding
A	0042995	cellular_component	cell projection
A	0043025	cellular_component	neuronal cell body
A	0043065	biological_process	positive regulation of apoptotic process
A	0043204	cellular_component	perikaryon
A	0043679	cellular_component	axon terminus
A	0050819	biological_process	negative regulation of coagulation
A	0051592	biological_process	response to calcium ion
A	0070588	biological_process	calcium ion transmembrane transport
A	0071284	biological_process	cellular response to lead ion
A	0072563	cellular_component	endothelial microparticle
A	0097066	biological_process	response to thyroid hormone
A	0097211	biological_process	cellular response to gonadotropin-releasing hormone
A	1901317	biological_process	regulation of flagellated sperm motility
A	1902721	biological_process	negative regulation of prolactin secretion

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA A 401

Chain	Residue
A	MET26
A	GLY28
A	GLY30
A	GLU70
A	HOH525
A	HOH599

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CA A 402

Chain	Residue
A	HOH600
A	LYS68
A	LEU71
A	GLU76

---

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA A 407

Chain	Residue
A	GLY181
A	LYS184
A	GLY186
A	GLU226
A	HOH541
A	HOH603

---

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CA A 408

Chain	Residue
A	ASP224
A	THR227
A	GLU232
A	HOH608

---

site_id	AC5
Number of Residues	7
Details	BINDING SITE FOR RESIDUE CA A 410

Chain	Residue
A	MET257
A	LYS258
A	GLY259
A	ALA260
A	GLY261
A	ASP301
A	HOH609

---

site_id	AC6
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 A 450

Chain	Residue
A	ARG23
A	MET26
A	LYS27
A	GLY28
A	ARG61
A	HOH501
A	HOH599

---

Functional Information from PROSITE/UniProt

site_id	PS00223
Number of Residues	53
Details	ANNEXIN_1 Annexin repeat signature. GTdedsilnlLtaRsnaQrqQiaeeFktlfgrdLvndMkselkGkfeklIvaL

Chain	Residue	Details
A	GLY30-LEU82
A	GLY102-LEU154
A	GLY186-VAL238
A	GLY261-LEU313

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	71
Details	Repeat: {"description":"Annexin 1","evidences":[{"source":"PROSITE-ProRule","id":"PRU01245","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	71
Details	Repeat: {"description":"Annexin 2","evidences":[{"source":"PROSITE-ProRule","id":"PRU01245","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	72
Details	Repeat: {"description":"Annexin 3","evidences":[{"source":"PROSITE-ProRule","id":"PRU01245","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	71
Details	Repeat: {"description":"Annexin 4","evidences":[{"source":"PROSITE-ProRule","id":"PRU01245","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	6
Details	Motif: {"description":"[IL]-x-C-x-x-[DE] motif","evidences":[{"source":"UniProtKB","id":"P08758","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	Modified residue: {"description":"N-acetylalanine","evidences":[{"source":"PubMed","id":"7583670","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"22673903","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	5
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"P08758","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	1
Details	Modified residue: {"description":"N6-succinyllysine","evidences":[{"source":"UniProtKB","id":"P48036","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate","evidences":[{"source":"UniProtKB","id":"P08758","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---