1BC3

RECOMBINANT RAT ANNEXIN V, TRIPLE MUTANT (T72K, S144K, S228K)

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1995-08
Detector	RIGAKU RAXIS
Spacegroup name	H 3
Unit cell lengths	157.340, 157.340, 37.590
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.950
R-factor	0.211 *
Rwork	0.259
R-free	0.25900 *
Structure solution method	DIFFERENCE FOURIER
RMSD bond length	0.005
RMSD bond angle	22.019 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000
High resolution limit [Å]	1.950
Rmerge	0.110 *
Number of reflections	21029
Completeness [%]	83.7
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8.2	17 *	PROTEIN CRYSTALLIZED FROM AMMONIUM SULFATE, 20MM CACL2, 50MM HEPPS, PH 8.2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	14 (mg/ml)
2	1	drop	calcium	20 (mM)
3	1	reservoir	ammonium sulfate	34-40 (%sat)
4	1	reservoir	HEPPS	50 (mM)
5	1	reservoir		0.02 (%)
6	1	reservoir	dithiothreitol	2 (mM)
7	1	reservoir		10 (mM)