1ARO

T7 RNA POLYMERASE COMPLEXED WITH T7 LYSOZYME

Functional Information from GO Data

Chain	GOid	namespace	contents
L	0008270	molecular_function	zinc ion binding
L	0008745	molecular_function	N-acetylmuramoyl-L-alanine amidase activity
L	0009253	biological_process	peptidoglycan catabolic process
L	0016787	molecular_function	hydrolase activity
L	0030430	cellular_component	host cell cytoplasm
L	0031640	biological_process	killing of cells of another organism
L	0032897	biological_process	negative regulation of viral transcription
L	0042742	biological_process	defense response to bacterium
L	0044659	biological_process	viral release from host cell by cytolysis
L	0046872	molecular_function	metal ion binding
P	0000428	cellular_component	DNA-directed RNA polymerase complex
P	0003677	molecular_function	DNA binding
P	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
P	0006351	biological_process	DNA-templated transcription
P	0016779	molecular_function	nucleotidyltransferase activity
P	0019083	biological_process	viral transcription
P	0034062	molecular_function	5'-3' RNA polymerase activity
P	0039695	biological_process	DNA-templated viral transcription

Functional Information from PDB Data

site_id	AC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE HG L 903

Chain	Residue
L	CYS1018
L	SER1019
L	CYS1130

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site_id	AC2
Number of Residues	2
Details	BINDING SITE FOR RESIDUE HG P 904

Chain	Residue
P	CYS125
P	ALA140

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site_id	AC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE HG P 905

Chain	Residue
P	GLU517
P	TYR528
P	CYS530

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site_id	AC4
Number of Residues	2
Details	BINDING SITE FOR RESIDUE HG P 906

Chain	Residue
P	CYS510
P	CYS467

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site_id	AC5
Number of Residues	1
Details	BINDING SITE FOR RESIDUE HG P 907

Chain	Residue
P	MET313

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site_id	AC6
Number of Residues	1
Details	BINDING SITE FOR RESIDUE HG P 908

Chain	Residue
P	MET401

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site_id	AC7
Number of Residues	2
Details	BINDING SITE FOR RESIDUE HG P 909

Chain	Residue
P	SER539
P	CYS540

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Functional Information from PROSITE/UniProt

site_id	PS00489
Number of Residues	15
Details	RNA_POL_PHAGE_2 Bacteriophage-type RNA polymerase family active site signature 2. VtRsvtKrsVMTlaY

Chain	Residue	Details
P	VAL625-TYR639

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site_id	PS00900
Number of Residues	12
Details	RNA_POL_PHAGE_1 Bacteriophage-type RNA polymerase family active site signature 1. PLafDGSCSGIQ

Chain	Residue	Details
P	PRO533-GLN544

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	3
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_04111, ECO:0000269|PubMed:8171031, ECO:0007744|PDB:1LBA

Chain	Residue	Details
L	CYS1018
L	HIS1123
L	PRO1131

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site_id	SWS_FT_FI2
Number of Residues	1
Details	SITE: Essential for amidase activity and zinc hydrate coordination => ECO:0000255|HAMAP-Rule:MF_04111, ECO:0000269|PubMed:8171031

Chain	Residue	Details
L	HIS1047

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site_id	SWS_FT_FI3
Number of Residues	1
Details	SITE: Important for catalytic activity => ECO:0000269|PubMed:8171031, ECO:0007744|PDB:1LBA

Chain	Residue	Details
L	ALA1129

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Catalytic Information from CSA

site_id	CSA1
Number of Residues	2
Details	Annotated By Reference To The Literature 1lba

Chain	Residue	Details
L	TYR1046
L	LYS1128

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site_id	CSA2
Number of Residues	1
Details	Annotated By Reference To The Literature 1lba

Chain	Residue	Details
P	LYS631

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site_id	MCSA1
Number of Residues	5
Details	M-CSA 586

Chain	Residue	Details
L	CYS1018
L	HIS1047	proton shuttle (general acid/base)
L	HIS1123
L	ALA1129	electrostatic stabiliser
L	PRO1131

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