1ARO
T7 RNA POLYMERASE COMPLEXED WITH T7 LYSOZYME
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-10 |
| Detector | FUJI |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 273.385, 95.612, 63.582 |
| Unit cell angles | 90.00, 101.40, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| R-factor | 0.262 |
| Rwork | 0.262 |
| R-free | 0.30900 |
| Structure solution method | MIR |
| RMSD bond length | 0.012 * |
| RMSD bond angle | 1.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.096 | 0.440 |
| Number of reflections | 38310 | |
| <I/σ(I)> | 13.37 | 2 |
| Completeness [%] | 93.9 | 76.8 |
| Redundancy | 2.2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7 | 12 * | Jeruzalmi, D., (1997) J. Mol. Biol., 274, 748. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 10 | 1 | drop | 0.02 (%) | ||
| 11 | 1 | reservoir | water | deionized, 0.500ml | |
| 2 | 1 | drop | Tris-HCl | 2.5 (mM) | |
| 3 | 1 | drop | potassium phosphate | 5 (mM) | |
| 4 | 1 | drop | 60 (mM) | ||
| 5 | 1 | drop | 25 (mM) | ||
| 6 | 1 | drop | sarcosine | 12-15 (%) | |
| 7 | 1 | drop | dithiothreitol | 1 (mM) | |
| 8 | 1 | drop | Na3 EDTA | 1 (mM) | |
| 9 | 1 | drop | beta-hexylglucoside | 0.05 (%(v/v)) |
