1ARO
T7 RNA POLYMERASE COMPLEXED WITH T7 LYSOZYME
Experimental procedure
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F2 |
Synchrotron site | CHESS |
Beamline | F2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1993-10 |
Detector | FUJI |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 273.385, 95.612, 63.582 |
Unit cell angles | 90.00, 101.40, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.800 |
R-factor | 0.262 |
Rwork | 0.262 |
R-free | 0.30900 |
Structure solution method | MIR |
RMSD bond length | 0.012 * |
RMSD bond angle | 1.800 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.096 | 0.440 |
Number of reflections | 38310 | |
<I/σ(I)> | 13.37 | 2 |
Completeness [%] | 93.9 | 76.8 |
Redundancy | 2.2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7 | 12 * | Jeruzalmi, D., (1997) J. Mol. Biol., 274, 748. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 30 (mg/ml) | |
10 | 1 | drop | 0.02 (%) | ||
11 | 1 | reservoir | water | deionized, 0.500ml | |
2 | 1 | drop | Tris-HCl | 2.5 (mM) | |
3 | 1 | drop | potassium phosphate | 5 (mM) | |
4 | 1 | drop | 60 (mM) | ||
5 | 1 | drop | 25 (mM) | ||
6 | 1 | drop | sarcosine | 12-15 (%) | |
7 | 1 | drop | dithiothreitol | 1 (mM) | |
8 | 1 | drop | Na3 EDTA | 1 (mM) | |
9 | 1 | drop | beta-hexylglucoside | 0.05 (%(v/v)) |