1A3X

PYRUVATE KINASE FROM SACCHAROMYCES CEREVISIAE COMPLEXED WITH PG, MN2+ AND K+

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0000287	molecular_function	magnesium ion binding
A	0003824	molecular_function	catalytic activity
A	0004743	molecular_function	pyruvate kinase activity
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005886	cellular_component	plasma membrane
A	0006090	biological_process	pyruvate metabolic process
A	0006096	biological_process	glycolytic process
A	0008152	biological_process	metabolic process
A	0016301	molecular_function	kinase activity
A	0016310	biological_process	phosphorylation
A	0016740	molecular_function	transferase activity
A	0030955	molecular_function	potassium ion binding
A	0032787	biological_process	monocarboxylic acid metabolic process
A	0032869	biological_process	cellular response to insulin stimulus
A	0046872	molecular_function	metal ion binding
B	0000166	molecular_function	nucleotide binding
B	0000287	molecular_function	magnesium ion binding
B	0003824	molecular_function	catalytic activity
B	0004743	molecular_function	pyruvate kinase activity
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0005886	cellular_component	plasma membrane
B	0006090	biological_process	pyruvate metabolic process
B	0006096	biological_process	glycolytic process
B	0008152	biological_process	metabolic process
B	0016301	molecular_function	kinase activity
B	0016310	biological_process	phosphorylation
B	0016740	molecular_function	transferase activity
B	0030955	molecular_function	potassium ion binding
B	0032787	biological_process	monocarboxylic acid metabolic process
B	0032869	biological_process	cellular response to insulin stimulus
B	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	10
Details	BINDING SITE FOR RESIDUE PGA A 1005

Chain	Residue
A	ARG49
A	K1002
A	LYS240
A	GLU242
A	ALA263
A	ARG264
A	GLY265
A	ASP266
A	THR298
A	MN1001

---

site_id	AC2
Number of Residues	12
Details	BINDING SITE FOR RESIDUE PGA B 1006

Chain	Residue
B	ARG49
B	SER213
B	LYS240
B	GLU242
B	ALA263
B	ARG264
B	GLY265
B	ASP266
B	ALA297
B	THR298
B	MN1003
B	K1004

---

site_id	AC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MN A 1001

Chain	Residue
A	GLU242
A	ASP266
A	PGA1005

---

site_id	AC4
Number of Residues	7
Details	BINDING SITE FOR RESIDUE K A 1002

Chain	Residue
A	ASN51
A	SER53
A	ASP84
A	THR85
A	SER213
A	LYS240
A	PGA1005

---

site_id	AC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MN B 1003

Chain	Residue
B	GLU242
B	ASP266
B	PGA1006

---

site_id	AC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE K B 1004

Chain	Residue
B	ASN51
B	SER53
B	ASP84
B	THR85
B	SER213
B	PGA1006

---

Functional Information from PROSITE/UniProt

site_id	PS00110
Number of Residues	13
Details	PYRUVATE_KINASE Pyruvate kinase active site signature. VkIIVKIENqQGV

Chain	Residue	Details
A	VAL235-VAL247

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:9519410, ECO:0007744|PDB:1A3X

Chain	Residue	Details
A	ARG49
A	LYS240
B	ARG49
B	LYS240

---

site_id	SWS_FT_FI2
Number of Residues	20
Details	BINDING: BINDING => ECO:0000269|PubMed:9519410, ECO:0007744|PDB:1A3W, ECO:0007744|PDB:1A3X

Chain	Residue	Details
A	ASN51
A	GLY484
B	ASN51
B	SER53
B	ASP84
B	THR85
B	GLU242
B	GLY265
B	THR298
B	TRP452
B	ARG459
A	SER53
B	GLY484
A	ASP84
A	THR85
A	GLU242
A	GLY265
A	THR298
A	TRP452
A	ARG459

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	BINDING: BINDING => ECO:0000250|UniProtKB:P14618

Chain	Residue	Details
A	ARG91
A	LYS177
B	ARG91
B	LYS177

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:9519410, ECO:0007744|PDB:1A3W

Chain	Residue	Details
A	ASP266
A	SER402
B	ASP266
B	SER402

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	SITE: Transition state stabilizer => ECO:0000269|PubMed:10413488

Chain	Residue	Details
A	LYS240
B	LYS240

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	MOD_RES: N-acetylserine => ECO:0007744|PubMed:17287358

Chain	Residue	Details
A	SER2
B	SER2

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:17330950, ECO:0007744|PubMed:19779198

Chain	Residue	Details
A	SER9
B	SER9

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:18407956, ECO:0007744|PubMed:19779198

Chain	Residue	Details
A	SER16
B	SER16

---

site_id	SWS_FT_FI9
Number of Residues	4
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:19779198

Chain	Residue	Details
A	THR31
A	THR184
B	THR31
B	THR184

---

site_id	SWS_FT_FI10
Number of Residues	6
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:19779198

Chain	Residue	Details
A	SER70
A	SER316
A	SER450
B	SER70
B	SER316
B	SER450

---

site_id	SWS_FT_FI11
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:17287358

Chain	Residue	Details
A	SER213
B	SER213

---

site_id	SWS_FT_FI12
Number of Residues	2
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:17330950, ECO:0007744|PubMed:19779198

Chain	Residue	Details
A	THR478
B	THR478

---

site_id	SWS_FT_FI13
Number of Residues	8
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0007744|PubMed:22106047

Chain	Residue	Details
A	LYS204
A	LYS255
A	LYS446
B	LYS204
B	LYS255
B	LYS446

---