1A3X
PYRUVATE KINASE FROM SACCHAROMYCES CEREVISIAE COMPLEXED WITH PG, MN2+ AND K+
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 286 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-01-08 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.300, 106.400, 105.500 |
| Unit cell angles | 90.00, 110.80, 90.00 |
Refinement procedure
| Resolution | 100.000 - 3.000 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.34100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pkn |
| RMSD bond length | 0.013 |
| RMSD bond angle | 24.480 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 100.000 |
| High resolution limit [Å] | 3.000 |
| Rmerge | 0.108 * |
| Total number of observations | 94232 * |
| Number of reflections | 19294 |
| Completeness [%] | 91.0 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 4 * | 28-33% (W/V) PEG 8000, 200 MM SODIUM ACETATE, 100MM SODIUM CACODYLATE PH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4-12 (mg/ml) | |
| 2 | 1 | drop | PG | 10 (mM) | |
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | drop | 200 (mM) | ||
| 5 | 1 | drop | FBP | 10 (mM) | |
| 6 | 1 | reservoir | PEG8000 | 34-36 (%(w/v)) | |
| 7 | 1 | reservoir | sodium acetate | 50 (mM) | |
| 8 | 1 | reservoir | sodium cacodylate | 100 (mM) |
