1A3X
PYRUVATE KINASE FROM SACCHAROMYCES CEREVISIAE COMPLEXED WITH PG, MN2+ AND K+
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 286 |
Detector technology | IMAGE PLATE |
Collection date | 1994-01-08 |
Detector | RIGAKU RAXIS II |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 108.300, 106.400, 105.500 |
Unit cell angles | 90.00, 110.80, 90.00 |
Refinement procedure
Resolution | 100.000 - 3.000 |
R-factor | 0.227 |
Rwork | 0.227 |
R-free | 0.34100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pkn |
RMSD bond length | 0.013 |
RMSD bond angle | 24.480 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 100.000 |
High resolution limit [Å] | 3.000 |
Rmerge | 0.108 * |
Total number of observations | 94232 * |
Number of reflections | 19294 |
Completeness [%] | 91.0 |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.5 | 4 * | 28-33% (W/V) PEG 8000, 200 MM SODIUM ACETATE, 100MM SODIUM CACODYLATE PH 6.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4-12 (mg/ml) | |
2 | 1 | drop | PG | 10 (mM) | |
3 | 1 | drop | 10 (mM) | ||
4 | 1 | drop | 200 (mM) | ||
5 | 1 | drop | FBP | 10 (mM) | |
6 | 1 | reservoir | PEG8000 | 34-36 (%(w/v)) | |
7 | 1 | reservoir | sodium acetate | 50 (mM) | |
8 | 1 | reservoir | sodium cacodylate | 100 (mM) |