9ZVU

The ubiquitin-associated domain of human thirty-eight negative kinase-1 rigidly fused to a double trigger variant of the 1TEL crystallization chaperone, alternate crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-11-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	38.213, 79.367, 119.173
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.590 - 1.964
R-factor	0.2127
Rwork	0.211
R-free	0.24690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.522
Data reduction software	autoPROC
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.590	2.035
High resolution limit [Å]	1.964	1.964
Rmerge	0.130	2.288
Rmeas	0.136	2.404
Rpim	0.040	0.727
Number of reflections	26655	2619
<I/σ(I)>	12.34	1.35
Completeness [%]	99.9	99.81
Redundancy	11.1	10.6
CC(1/2)	0.999	0.521

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	0.2M Sodium Tartrate, 16% PEG 3350 Monodisperse