9ZRN

Neutralizing W014 Fab antibody fragment in complex with West Nile Virus EDIII

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-02-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	41.102, 122.092, 54.075
Unit cell angles	90.00, 106.33, 90.00

Refinement procedure

Resolution	39.540 - 2.100
R-factor	0.2086
Rwork	0.207
R-free	0.24290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.795
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.540	2.160
High resolution limit [Å]	2.100	2.100
Number of reflections	29459	2272
<I/σ(I)>	5.4
Completeness [%]	98.3
Redundancy	3.6
CC(1/2)	0.989	0.478

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	20% w/v 2-Propanol, 20% w/v PEGME2000, 0.1 MES