9ZP3
E. coli RNA polymerase elongation complex containing the unnatural dP:Z*TP base pair in a trigger-loop-closed conformation.
This is a non-PDB format compatible entry.
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | TFS FALCON 4i (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.75 |
| Software | cryoSPARC,PHENIX |
| CTF correction | NONE |
