9YRO

Cryo-EM structure of SARS-CoV-2 nsp10-nsp14 (E191A) in complex with T20P14-S complex, monomeric form

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK IV

EM Data Collection

Microscope	TFS KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2.7
Energy Filter	TFS Selectris X
Detector	TFS FALCON 4i (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	2.55
Software	cryoSPARC,EPU,ISOLDE,PHENIX
Symmetry	C1
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION