9YKN
The structure of the cardiac native crossbridge in the rigor state, myosin heads with essential and regulatory light chains bound to actin molecules 6 and 7
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 5.3 |
| Number of Particle | 5235506 |
| Software | RELION,UCSF Chimera,PHENIX,EPU,CTFFIND,ISOLDE,MDFF |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
