9YD1

Crystal structure of BF DNA polymerase F710Y mutant bound to tetrahydrofuran and dATP

Replaces:  6UEU

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2006-07-06
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	93.875, 109.068, 150.096
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.987 - 1.800
R-factor	0.2024
Rwork	0.200
R-free	0.24230
RMSD bond length	0.018
RMSD bond angle	1.347
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	REFMAC
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.987	44.987	1.900
High resolution limit [Å]	1.800	5.340	1.800
Rmerge	0.078	0.037	0.595
Rmeas	0.089	0.041	0.678
Total number of observations	632948
Number of reflections	138448	5091	21082
<I/σ(I)>	12.27	35.88	2.79
Completeness [%]	96.9	87.3	99.7
Redundancy	4.572	4.787	4.468

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	290	50% (v/v) saturated ammonium sulfate, 20 mM MgSO4, 100 mM MES, 1% MPD